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The serial cultivation of human diploid cell strains. Exp Cell Res (1961)
| Citations: | 495 - 2 self |
BibTeX
@MISC{Hayflick61theserial,
author = {L. Hayflick and P. S. Moorhead},
title = {The serial cultivation of human diploid cell strains. Exp Cell Res},
year = {1961}
}
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Abstract
ONLY limited success has been obtained in developing strains of human cells that can be cultivated for long periods of time in vitro and that still preserve the diploid chromosomal configuration [41, 47, 48, 58, 591. Indeed, heteroploidy may be a necessary corollary or even the cause of the altera-tion of primary or diploid cells in vitro to the status of a cell line. Such changes in chromosome number appear to be independent of the type of primary tissue since they have been observed in cells derived from both normal and malignant tissue [4, 22, 23, 311. These cell lines, of which over two hundred have been reported in the literature, have serious limitations for many kinds of biological studies. Chief among these is the exclusion of their use for the production of human virus vaccines. This limitation is based on the supposition that such hetero-ploid cell lines, whether of normal or malignant origin, share many of the properties of malignant cells [29, 30, 371. This objection would be even more important if viruses played a role in human neoplasia. In general, if
Keyphrases
exp cell re human diploid cell strain serial cultivation cell line long period malignant origin limited success human virus vaccine hetero-ploid cell line necessary corollary many kind primary tissue serious limitation diploid cell malignant tissue chromosome number malignant cell diploid chromosomal configuration human neoplasia human cell biological study
