BibTeX
@MISC{Martinoli_supplementalexperimental,
author = {Chiara Martinoli and Andrea Chiavelli and Maria Rescigno},
title = {Supplemental Experimental Procedures},
year = {}
}
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Abstract
with DNAse (Qiagen) and retro-transcribed with oligo-dT primers (Invitrogen) and Powerscript Reverse Transcriptase (Clontech). Post-switch α transcripts were amplified using the primers ImF and CaR (Muramatsu et al., 2000) in a 40 cycles of PCR. Circular transcripts for γ1 were amplified as described (Kinoshita et al., 2001). Quantitative RT-PCR for pIgR Expression Small intestines were collected from naïve or infected mice and flushed with sterile PBS. PP were removed and intestines were opened longitudinally, washed once with DTT (1mM in PBS) and incubated with cold HEPES/HBSS solution (150mM HEPES, Ca2+ and Mg2+ free HBSS, pH 7.2) for 10 minutes. Epithelial cells were detached by a 20 minutes 37°C incubation in EDTA/HBSS
