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The APETALA-2-Like Transcription Factor OsAP2-39 Controls Key Interactions between Abscisic Acid and
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BibTeX
@MISC{Rice_theapetala-2-like,
author = {Gibberellin In Rice and Mahmoud W. Yaish and Ashraf El-kereamy and Tong Zhu and Perrin H. Beatty and Allen G. Good and Yong-mei Bi and Steven J. Rothstein},
title = {The APETALA-2-Like Transcription Factor OsAP2-39 Controls Key Interactions between Abscisic Acid and},
year = {}
}
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Abstract
The interaction between phytohormones is an important mechanism which controls growth and developmental processes in plants. Deciphering these interactions is a crucial step in helping to develop crops with enhanced yield and resistance to environmental stresses. Controlling the expression level of OsAP2-39 which includes an APETALA 2 (AP2) domain leads to phenotypic changes in rice. Overexpression of OsAP2-39 leads to a reduction in yield by decreasing the biomass and the number of seeds in the transgenic rice lines. Global transcriptome analysis of the OsAP2-39 overexpression transgenic rice revealed the upregulation of a key Abscisic Acid (ABA) biosynthetic gene OsNCED-I which codes for 9-cis-epoxycarotenoid dioxygenase and leads to an increase in the endogenous ABA level. In addition to OsNCED-1, the gene expression analysis revealed the upregulation of a gene that codes for the Elongation of Upper most Internode (EUI) protein, an enzyme that catalyzes 16a, 17-epoxidation of non-13-hydroxylated GAs, which has been shown to deactivate gibberellins (GAs) in rice. The exogenous application of GA restores the wild-type phenotype in the transgenic line and ABA application induces the expression of EUI and suppresses the expression of OsAP2-39 in the wild-type line. These observations clarify the antagonistic relationship between ABA and GA and illustrate a mechanism that leads to homeostasis of these hormones. In vivo and in vitro analysis showed that the expression of both OsNCED-1 and EUI are directly controlled by OsAP2-39. Together, these results reveal a novel mechanism for the control of the ABA/GA balance in rice which is regulated by OsAP2-
Keyphrases
abscisic acid non-13-hydroxylated gas 9-cis-epoxycarotenoid dioxygenase important mechanism antagonistic relationship transgenic rice line developmental process aba application biosynthetic gene osnced-i endogenous aba level key abscisic acid wild-type line global transcriptome analysis wild-type phenotype crucial step enhanced yield aba ga balance environmental stress expression level transgenic line gene expression analysis exogenous application novel mechanism vitro analysis osap2-39 overexpression transgenic rice
