DMCA
Hyaluronidase Treatment, Collagen Fibril Packing, and Normal Transparency in Rabbit Corneas
BibTeX
@MISC{Alba_hyaluronidasetreatment,,
author = {S. A. Alba and Ms H. Karageozian},
title = {Hyaluronidase Treatment, Collagen Fibril Packing, and Normal Transparency in Rabbit Corneas},
year = {}
}
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Abstract
PURPOSE: Hyaluronidase treatment is the initial step of corneaplasty, a treatment under development that induces stromal softening and involves the application of a custom designed forming lens to achieve modification of refractive error. The purpose of this investigation was to examine changes in the arrangement of stromal collagen fibrils after hyaluronidase treatment. METHODS: Rabbit corneas were evaluated by slit-lamp microscopy at 0, 2 and 7 days after treatment and haze was assessed by subjective observation. Molecular and interfibrillar Bragg spacing of corneal collagen were measured from synchrotron x-ray scattering patterns. Transmission electron microscopy and digital image analysis were used to calculate radial distribution functions from the positions of collagen fibrils. The calculated fibril sizes and positions were also used to predict the transmission of visible light through these corneas. RESULTS: Hyaluronidase-treated corneas were shown to have a decreased interfibr...
Keyphrases
hyaluronidase treatment rabbit cornea collagen fibril packing normal transparency digital image analysis initial step radial distribution function synchrotron x-ray scattering pattern stromal collagen fibril refractive error hyaluronidase-treated cornea corneal collagen slit-lamp microscopy subjective observation visible light collagen fibril calculated fibril size interfibrillar bragg spacing stromal softening transmission electron microscopy
