DMCA
Negative staining of thinly spread cells and associated virus (1963)
| Venue: | J. Cell Biol |
| Citations: | 8 - 0 self |
BibTeX
@ARTICLE{Parsons63negativestaining,
author = {Donald F. Parsons and From Ontario},
title = {Negative staining of thinly spread cells and associated virus},
journal = {J. Cell Biol},
year = {1963}
}
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Abstract
The first demonstration of intracytoplasmic virus by negative staining was that of Horne and Nagington (9) who subjected poliovirus-infected cells to freezing and thawing and negatively stained a suspension of the fragments. Dales (5) examined the attachment of some viruses to the cell surface by the negative staining of whole mounts of cells subjected to osmotic shock. Whittaker and Horne (15) examined normal cell components by the negative staining of fractions of tissue homogenates separated by centrifugation. Methods of negative staining of thin sections of frozen or frozen-dried tissue have been developed by Fern(tndez-Mor~tn (7). Almeida and Howatson (1, 2) have recently developed a
