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Connexin43: a protein from rat heart homologous to a gap junction protein from liver (1987)
| Venue: | J. Cell |
| Citations: | 59 - 7 self |
BibTeX
@ARTICLE{Beyer87connexin43:a,
author = {Eric C. Beyer and David L. Paul and Daniel A. Goodenough},
title = {Connexin43: a protein from rat heart homologous to a gap junction protein from liver},
journal = {J. Cell},
year = {1987},
pages = {105--2621}
}
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Abstract
Abstract. Northern blot analysis of rat heart mRNA probed with a eDNA coding for the principal polypeptide of rat liver gap junctions demonstrated a 3.0-kb band. This band was observed only after hybridization and washing using low stringency conditions; high stringency conditions abolished the hybridization. A rat heart eDNA library was screened with the same eDNA probe under the permissive hybridization conditions, and a single positive clone identified and purified. The clone contained a 220-bp insert, which showed 55 % homology to the original eDNA probe near the 5 ' end. The 220-bp eDNA was used to rescreen a heart eDNA library under high stringency conditions, and three additional cDNAs that together spanned 2,768 bp were isolated. This composite eDNA
Keyphrases
gap junction protein rat heart high stringency condition single positive clone composite edna rat heart mrna rat heart edna library 0-kb band heart edna library additional cdna northern blot analysis principal polypeptide rat liver gap junction edna probe original edna probe 220-bp edna permissive hybridization condition low stringency condition 220-bp insert
