BibTeX
@MISC{_in40,
author = {},
title = {in 40 ml of AGK 200 [10 mM Hepes (pH},
year = {}
}
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Abstract
PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY98 with pG1-PRP24-Pya as sole PRP24 gene), were harvested in late logarithmic phase. Whole cell extract was prepared (15), and 150 mg of each was subjected to 30 to 55 % ammonium sulfate precipitation. The precipitates were resuspended
