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An analysis of the feasibility of short read sequencing (2005)
| Citations: | 23 - 1 self |
BibTeX
@MISC{Whiteford05ananalysis,
author = {Nava Whiteford and Niall Haslam and Gerald Weber and Adam Prügel-bennett and Jonathan W. Essex and Peter L. Roach and Mark Bradley and Cameron Neylon},
title = {An analysis of the feasibility of short read sequencing},
year = {2005}
}
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Abstract
Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that resequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths of 20–30 nt, and that reads of 50 nt can provide reconstructed contigs (a contiguous fragment of sequence data) of 1000 nt and greater that cover 80 % of human chromosome 1.
