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www.plantroot.org RootLM: a simple color image analysis program for length measurement of primary roots in Arabidopsis (2006)
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@MISC{Qi06www.plantroot.orgrootlm:,
author = {Xiaojun Qi and Ji Qi and Yajun Wu},
title = {www.plantroot.org RootLM: a simple color image analysis program for length measurement of primary roots in Arabidopsis},
year = {2006}
}
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Abstract
Abstract: The Arabidopsis root is a model system for studying root growth, development, and response to environmental stresses. Due to its small size, many Arabidopsis seedlings can be cultured in a single nutrient-containing agar plate. Two of the common parameters used to evaluate root growth are the root length and the elongation rate. Various methods including manual measurement and digital image analysis can be used to measure the root length. In this paper, we report the development of a simple color image analysis program, RootLM, in combination with a modified marking technique of root growth on the surface of a Petri dish, for length measurement of primary roots. Our method provides an alternative tool to quickly generate accurate and reproducible root growth measurement with a minimum equipment requirement. In addition, the program is free for public download.
Keyphrases
length measurement simple color image analysis program primary root root growth org rootlm root length various method reproducible root growth measurement alternative tool digital image analysis single nutrient-containing agar plate elongation rate environmental stress minimum equipment requirement modified marking technique model system public download arabidopsis root petri dish many arabidopsis seedling manual measurement common parameter small size
