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Functional discovery via a compendium of expression profiles. (2000)
| Venue: | Cell, |
| Citations: | 546 - 9 self |
BibTeX
@ARTICLE{Hughes00functionaldiscovery,
author = {Timothy R Hughes and Matthew J Marton and Allan R Jones and Christopher J Roberts and Roland Stoughton and Christopher D Armour and Holly A Bennett and Ernest Coffey and Hongyue Dai and Ross-Macdonald and Yudong D He and Matthew J Kidd and Amy M King and Michael R Meyer and David Slade and Pek Y Lum and Sergey B Stepaniants and Daniel D Shoemaker and Julian Simon and Martin Bard},
title = {Functional discovery via a compendium of expression profiles.},
journal = {Cell,},
year = {2000},
pages = {109--126}
}
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Abstract
provided that the cellular transcriptional response to frames encode proteins required for sterol metabodisruption of different steps in the same pathway is lism, cell wall function, mitochondrial respiration, or similar, and that there are sufficiently unique transcripprotein synthesis. We also show that the compendium tional responses to the perturbation of most cellular can be used to characterize pharmacological perturpathways, systematic characterization of novel mutants bations by identifying a novel target of the commonly could be carried out with a single genome-wide expresused drug dyclonine. sion measurement. Using a comprehensive database of reference profiles, the pathway(s) perturbed by an Introduction uncharacterized mutation would be ascertained by simply asking which expression patterns in the database Systematic approaches for identifying the biological its profile most strongly resembles, in a manner analofunctions of novel genes are needed to ensure rapid gous to fingerprinting. An appealing aspect of this approgress from genome sequence to directed experiproach is that it should be equally effective at determinmentation and applications. A similar need exists for ing the consequences of pharmaceutical treatments and methods to characterize biologically active compounds. disease states. A sufficiently large and diverse set of profiles obtained from different mutants, treatments, and conditions would also result in a relatively compreTo whom correspondence should be addressed (e-mail: sfriend@ hensive identification of coregulated transcript groups, rii.com). # These authors contributed equally to this work. allowing additional hypotheses to be drawn regarding
