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Arabidopsis E2Fc functions in cell division and is degraded by the ubiquitinSCF(AtSKP2) pathway in response to light (2002)
| Venue: | Plant Cell |
| Citations: | 23 - 4 self |
BibTeX
@ARTICLE{Pozo02arabidopsise2fc,
author = {Juan Carlos Del Pozo and Maria Beatrice Boniotti and Crisanto Gutierrez},
title = {Arabidopsis E2Fc functions in cell division and is degraded by the ubiquitinSCF(AtSKP2) pathway in response to light},
journal = {Plant Cell},
year = {2002},
pages = {3057--3071}
}
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Abstract
Selective ubiquitin-mediated proteolysis through the cell cycle controls the availability, and therefore the activity, of several cell proliferation proteins. E2F transcription factors play distinct roles in both proliferating and differentiated cells by regulating gene expression. Here, we report that Arabidopsis AtE2Fc is regulated by a balance between gene expression and ubiquitin-proteasome proteolysis. AtE2Fc degradation implicates the function of the E3 ubiquitin-ligase Skp1, Cullin, F-box (SCF AtSKP2) complex and seems to be dependent on cyclin-dependent kinase phosphorylation. In addition, we found that AtE2Fc degradation is triggered by light stimulation of dark-grown seedlings. Interestingly, the auxin response mutant axr1-12, in which RUB1 modification of the SCF component CUL1 is impaired, shows increased AtE2Fc protein levels, suggesting a dysfunction in the control of AtE2Fc stability. Likewise, overexpression of a stable form of the AtE2Fc protein negatively affects cell division and increases cell size. These effects are mediated, at least in part, by downregulating the cell cycle gene AtCDC6. The negative role of AtE2Fc in gene expression is further supported by the fact that AtE2Fc interacts with plant retinoblastoma-related protein, suggesting that AtE2Fc might form part of a repressor complex. We propose that AtE2Fc might play a role in cell division and during the transition from skotomorphogenesis to photomorphogenesis.
Keyphrases
cell division arabidopsis e2fc function gene expression ate2fc degradation ate2fc protein ate2fc stability auxin response mutant axr1-12 cell size cyclin-dependent kinase phosphorylation dark-grown seedling repressor complex selective ubiquitin-mediated proteolysis several cell proliferation protein plant retinoblastoma-related protein negative role e3 ubiquitin-ligase skp1 cell cycle differentiated cell stable form e2f transcription factor ubiquitin-proteasome proteolysis scf atskp2 rub1 modification arabidopsis ate2fc scf component cul1 cell cycle gene atcdc6 ate2fc protein level distinct role light stimulation
