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A simplified method for automated phosphorus analysis of serum and (1968)
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BibTeX
@MISC{Yee68asimplified,
author = {Hugh Y. Yee},
title = {A simplified method for automated phosphorus analysis of serum and},
year = {1968}
}
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Abstract
A simplified procedure utilizing ferrous ammonium sulfate-thiourea as the reductant for the determination of inorganic phosphate in serum and urine by the AutoAnalyzer is described. Elimination of the heating bath usually required for many phosphorus methods for color development yields an improved S.D. of ± 0.25 and facilitates troubleshooting during any apparatus malfunction. Comparison of results between the proposed method and that of Fiske and SubbaRow (1) is presented. Recoveries of added phosphorous in serum or urine are quantitative. N UMEROUS REDUCING AGENTS for the formation of molybdenum blue from the phosphomolybdate complex have been successfully used for the determination of inorganic phosphate. Perhaps the most widely used is the compound 1-amino-2-naphthol-4-sulfonic acid which was intro-duced by Fiske and SubbaRow (1). Other reducing agents employed in various methodologies are stanilous chloride (.2), ferrous sulfate (3, 4), p-semidine (5), and more recently citrate-arsenite (6) and ferrous ammonium sulfate (7). Experience has shown many of the reductants used for the analysis of inorganic phosphate have disadvantages, e.g., a short shelf life, a high blank, yielding unstable molybdenum blue colors, and requiring heat for color development. The AutoAnalyzer utilizes the method of Fiske and SubbaRow (i) as the choice. In practice this method works quite well, but has two minor shortcomings: (1) the use of a tempera-ture of 95#{176}for color development causes a frequent deterioration of tile bubble pattern, and (2) over a period of time, a deposition of molybdenum blue may occur within the heating coil. The former problem can be minimized by using a slightly lower temperature, 80-85#{176},for color development, and tile iatter problem by washing the system after analysis with 1 N NaOII via tile molybdale reagent line.
Keyphrases
color development simplified method inorganic phosphate phosphorus analysis molybdenum blue ferrous sulfate high blank heating coil improved s.d. phosphomolybdate complex frequent deterioration ferrous ammonium sulfate-thiourea tile molybdale reagent line simplified procedure unstable molybdenum blue color apparatus malfunction former problem minor shortcoming ferrous ammonium sulfate many phosphorus method tile bubble pattern iatter problem short shelf life umerous reducing agent color development yield various methodology compound 1-amino-2-naphthol-4-sulfonic acid stanilous chloride
