DMCA
Modified HPLC-Electrospray Ionization/ Mass Spectrometry Method for HbA1c Based on IFCC Reference Measurement Procedure
BibTeX
@MISC{_modifiedhplc-electrospray,
author = {},
title = {Modified HPLC-Electrospray Ionization/ Mass Spectrometry Method for HbA1c Based on IFCC Reference Measurement Procedure},
year = {}
}
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Abstract
is important in the management of diabetes. The IFCC reference measurement procedure for HbA1c is based on the ratio of glycated to nonglycated N-terminal hexapeptides of the -chains of hemoglobin after di-gestion with Glu-C endoproteinase. We developed a modification of the original reference measurement procedure with HPLC-electrospray ionization/mass spectrometry (ESI/MS). METHOD: We performed chromatographic separation of the hexapeptides using a C12 reversed-phase col-umn and a binary gradient system consisting of a mix-ture of H2O/acetonitrile/formic acid. RESULTS: Using this method, we obtained higher signal intensities and improved system stability compared with the reference measurement procedure. In the
Keyphrases
ifcc reference measurement procedure hplc-electrospray ionization mass spectrometry method improved system stability chromatographic separation original reference measurement procedure esi m c12 reversed-phase col-umn glu-c endoproteinase formic acid n-terminal hexapeptides hplc-electrospray ionization mass spectrometry reference measurement procedure binary gradient system signal intensity
