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CORTISONE-INDUCED ALTERATIONS IN MITOCHONDRIAL FUNCTION AND STRUCTURE
| Citations: | 3 - 0 self |
BibTeX
@MISC{Kimberg_cortisone-inducedalterations,
author = {Daniel V. Kimberg and Alden V. Loud and Joseph Wiener},
title = {CORTISONE-INDUCED ALTERATIONS IN MITOCHONDRIAL FUNCTION AND STRUCTURE},
year = {}
}
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Abstract
The effects of cortisone treatment on oxygen consumption, oxidative phosphorylation, and fine structure of rat liver mitochondria have been studied. Male rats weighing 125 g were treated for 6 days with 5 mg of cortisone acetate or isotonic saline. On the 7th day, sections of liver were excised and processed for light and electron microscopy. Mitochondrial respiration and oxidative phosphorylation were studied with mitochondria isolated from these livers. Cortisone treatment is responsible for a 14-40 % decrease in the amount of oxygen consumed per mg of mitochondrial protein when succinate, a-ketoglutarate, or j-hydroxybutyrate are used as substrates, or with ascorbate and N,N, N,NI-tetramethyl p-phenylenediamine as electron donors. In addition, oxidative phosphorylation is uncoupled with a lowering of the P:O ratios. Randomly selected liver cells have been analyzed by quantitative morphometric techniques. The average mitochondrial volume is increased fourfold in the peripheral and midzonal regions with a commensurate decrease in the number of mitochondria per cell. These alterations are present throughout the hepatic lobule, but are most marked in midzonal cells. The total mitochondrial volume per cell and the per cent of the
Keyphrases
oxidative phosphorylation cortisone-induced alteration mitochondrial function structure cortisone treatment cortisone acetate fine structure average mitochondrial volume midzonal cell mitochondrial respiration total mitochondrial volume electron microscopy quantitative morphometric technique 14-40 decrease isotonic saline per cent ni-tetramethyl p-phenylenediamine midzonal region electron donor oxygen consumption rat liver mitochondrion commensurate decrease liver cell hepatic lobule male rat 7th day mitochondrial protein
