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Cell membrane alignment along adhesive surfaces: contribution of active and passive cell processes (2003)
| Venue: | Biophys. J |
| Citations: | 13 - 0 self |
BibTeX
@ARTICLE{Pierres03cellmembrane,
author = {Anne Pierres and Emmanuelle Baloche and Dominique Touchard and Anne-marie Benoliel},
title = {Cell membrane alignment along adhesive surfaces: contribution of active and passive cell processes},
journal = {Biophys. J},
year = {2003},
pages = {2058--2070}
}
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Abstract
ABSTRACT Cell adhesion requires nanometer scale membrane alignment to allow contact between adhesion receptors. Little quantitative information is presently available on this important biological process. Here we present an interference reflection microscopic study of the initial interaction betweenmonocytic THP-1 cells and adhesive surfaces, with concomitant determination of cell deformability, using micropipette aspiration, and adhesiveness, using a laminar flow assay. We report that 1), during the first few minutes after contact, cells form irregular-shaped interaction zones reaching;100 mm2 with a margin extension velocity of 0.01–0.02 mm/s. This happens before the overall cell deformations usually defined as spreading. 2), These interference reflection microscopic-detected zones represent bona fide adhesion inasmuch as cells are not released by hydrodynamic forces. 3), Alignment is markedly decreased but not abolished by microfilament blockade with cytochalasin or even cell fixation with paraformaldehyde. 4), In contrast, exposing cells to hypotonic medium increased the rate of contact extension. 5), Contacts formed in presence of cytochalasin, after paraformaldehyde fixation or in hypotonic medium, were much more regular-shaped than controls and their extension matched cell deformability. 6), None of the aforementioned treatments altered adhesiveness to the surface. It is concluded that adhesive forces and passive membrane deformations are sufficient to generate initial cell alignment to adhesive surfaces, and this process is accelerated by spontaneous cytoskeletally-driven membrane motion.
Keyphrases
adhesive surface cell membrane alignment passive cell process hypotonic medium cell deformability interference reflection microscopic study concomitant determination overall cell deformation micropipette aspiration initial cell alignment spontaneous cytoskeletally-driven membrane motion laminar flow assay adhesive force contact extension nanometer scale membrane alignment aforementioned treatment passive membrane deformation interference reflection microscopic-detected zone abstract cell adhesion important biological process hydrodynamic force margin extension velocity little quantitative information first minute adhesion receptor bona fide adhesion irregular-shaped interaction zone paraformaldehyde fixation initial interaction betweenmonocytic thp-1 cell microfilament blockade
