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COMPASS: a tool for comparison of multiple protein alignments with assessment of statistical significance (2003)
| Venue: | J. Mol. Biol |
| Citations: | 125 - 33 self |
BibTeX
@ARTICLE{Sadreyev03compass:a,
author = {Ruslan Sadreyev and Nick Grishin and Howard Hughes Medical},
title = {COMPASS: a tool for comparison of multiple protein alignments with assessment of statistical significance},
journal = {J. Mol. Biol},
year = {2003},
pages = {317--336}
}
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Abstract
We present a novel method for the comparison of multiple protein align-ments with assessment of statistical significance (COMPASS). The method derives numerical profiles from alignments, constructs optimal local profile–profile alignments and analytically estimates E-values for the detected similarities. The scoring system and E-value calculation are based on a generalization of the PSI-BLAST approach to profile–sequence comparison, which is adapted for the profile–profile case. Tested along with existing methods for profile–sequence (PSI-BLAST) and profile– profile (prof_sim) comparison, COMPASS shows increased abilities for sensitive and selective detection of remote sequence similarities, as well as improved quality of local alignments. The method allows prediction of relationships between protein families in the PFAM database beyond the range of conventional methods. Two predicted relations with high sig-nificance are similarities between various Rossmann-type folds and between various helix-turn-helix-containing families. The potential value of COMPASS for structure/function predictions is illustrated by the detec-tion of an intricate homology between the DNA-binding domain of the CTF/NFI family and the MH1 domain of the Smad family.
Keyphrases
statistical significance multiple protein alignment local alignment various rossmann-type fold dna-binding domain e-value calculation mh1 domain numerical profile pfam database profile profile scoring system potential value intricate homology profile profile case high sig-nificance profile sequence ctf nfi family sequence comparison conventional method remote sequence similarity psi-blast approach smad family various helix-turn-helix-containing family structure function prediction multiple protein align-ments selective detection novel method detected similarity protein family
