DMCA
Transmembrane Molecules for Phylogenetic Analyses of Pathogenic Protists: Leishmania-Specific Informative Sites in Hydrophilic Loops of Trans- (2006)
BibTeX
@MISC{Reticulum06transmembranemolecules,
author = {Endoplasmic Reticulum and N-acetylglucosamine--phosphate Transferase},
title = {Transmembrane Molecules for Phylogenetic Analyses of Pathogenic Protists: Leishmania-Specific Informative Sites in Hydrophilic Loops of Trans-},
year = {2006}
}
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Abstract
A sequence database was created for the Leishmania N-acetylglucosamine-1-phosphate transferase (nagt) gene from 193 independent isolates. PCR products of this single-copy gene were analyzed for restriction fragment length polymorphism based on seven nagt sequences initially available. We subsequently sequenced 77 samples and found 19 new variants (genotypes). Alignment of all 26 nagt sequences is gap free, except for a single codon addition or deletion. Phylogenetic analyses of the sequences allow grouping the isolates into three subgenera, each consisting of recognized species complexes, i.e., subgenus Leishmania (L. amazonensis-L. mexicana, L. donovani-L. infantum, L. tropica, L. major, and L. turanica-L. gerbilli), subgenus Viannia (L. braziliensis, L. panamensis), and one unclassified (L. enriettii) species. This hierarchy of grouping is also supported by sequence analyses of selected samples for additional single-copy genes present on different chromosomes. Intraspecies divergence of nagt varies considerably with different species complexes. Interest-ingly, species complexes with less subspecies divergence are more widely distributed than those that are more divergent. The relevance of this to Leishmania evolutionary adaptation is discussed. Heterozygosity of subspe-cies variants contributes to intraspecies diversity, which is prominent in L. tropica but not in L. donovani-L. infantum. This disparity is thought to result from the genetic recombination of the respective species at
Keyphrases
phylogenetic analysis pathogenic protist transmembrane molecule leishmania-specific informative site hydrophilic loop nagt sequence genetic recombination subspecies divergence pcr product subgenus viannia different specie complex nagt varies single-copy gene subspe-cies variant new variant sequence database additional single-copy gene single codon addition leishmania evolutionary adaptation leishmania n-acetylglucosamine-1-phosphate transferase subgenus leishmania restriction fragment length polymorphism different chromosome intraspecies divergence independent isolates specie complex sequence analysis respective specie intraspecies diversity
