DMCA
Neuroimaging with Synchrotron-based Methods
BibTeX
@MISC{Kuehbacher_neuroimagingwith,
author = {M. Kuehbacher and B. Gruenewald and G. Falkenberg and T. Donath and J. Herzen and F. Beckmann and A. Kyriakopoulos},
title = {Neuroimaging with Synchrotron-based Methods},
year = {}
}
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Abstract
Golgi and Ramón y Cajal established the anatomy of the central nervous system by neuroimaging with staining techniques on fixed tissue. The modern functional brain imaging techniques are non-invasive and are applied on living brains. Methods like functional magnetic resonance imagery, positron emission tomography, electroencephalography, event related potential, optical imaging, magnetoencephalography belong to the toolbox of neuroimaging. Synchrotron-based tomography is on its way to enlarge this toolbox. Synchrotron radiation x-ray fluorescence analysis (SRXRF) allows the determination of the metal distribution in cryosections of tissues. Microprobe-synchrotron radiation X-ray fluorescence (micro-SRXRF) has been applied to determine the distribution of several metals and metalloids in cryo-sections of 10 µm thin tissue sections by scanning with a focused beam of synchrotron radiation at beamline L. The third spatial dimension of the elemental distribution pattern can be investigated by tomographic techniques. The results of our study with synchrotron radiation-based computered microtomography (SRµCT) in absorption contrast mode at beamline BW2 shows the importance of the right choice of the used animal model for the development of new neuroimaging methods. Animals with small brains are particularly useful models. We used honeybees, because their neuroanatomy is studied in great detail and we
Keyphrases
synchrotron-based method synchrotron radiation x-ray fluorescence analysis great detail functional magnetic resonance imagery tomographic technique focused beam microprobe-synchrotron radiation x-ray fluorescence animal model elemental distribution pattern magnetoencephalography belong several metal optical imaging sr ct central nervous system positron emission tomography beamline bw2 fixed tissue synchrotron-based tomography third spatial dimension thin tissue section modern functional brain imaging technique synchrotron radiation living brain absorption contrast mode useful model small brain right choice metal distribution
