BibTeX
@MISC{02acorrelation,
author = {},
title = {A Correlation between a Proteomic Evaluation and Conventional},
year = {2002}
}
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Abstract
4-Aminophenol (4-AP), D-serine, and cisplatin are established rodent nephrotoxins that damage proximal tubules within the renal cortex. Using high throughput 2D gel proteomics to profile protein changes in the plasma of compound-treated animals, we identified several markers of kidney toxicity. Male F344 and Alpk rats were treated with increasing doses of 4-AP, D-serine, or cisplatin, and plasma samples were collected over time. Control groups received saline or nontoxic isomers, L-serine, and transplatin. Plasma proteins that displayed dose- and temporal-dependent regulation in each study were further characterized by mass spectrometry to elucidate the protein identity. Several isoforms of the rat-specific T-kininogen protein were identified in each study. T-kininogen was elevated in the plasma of 4-AP-, D-serine-, and cisplatin-treated animals at early time points, returning to baseline levels 3 weeks after treatment. The protein was not elevated in the
Keyphrases
proteomic evaluation gel proteomics plasma sample mass spectrometry kidney toxicity proximal tubule temporal-dependent regulation male f344 rodent nephrotoxin several isoforms compound-treated animal several marker cisplatin-treated animal protein identity alpk rat protein change rat-specific t-kininogen protein high throughput early time point nontoxic isomer control group renal cortex
