M (2013) Single-tube multiplexed molecular detection of endemic porcine viruses in combination with background screening for transboundary diseases

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M (2013) Single-tube multiplexed molecular detection of endemic porcine viruses in combination with background screening for transboundary diseases

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by Kerstin Wernike , Bernd Hoffmann , Martin Beer

Venue:	Journal of clinical microbiology
Citations:	1 - 0 self

Citations

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207	Nidovirales: a new order comprising Coronaviridae and Arteriviridae. - Cavanagh - 1997 (Show Context) Citation Context ...us type 2 (PCV2) is common in countries with intensive swine production (1, 2). Infection with PRRSV, an enveloped positivestrand RNA virus that belongs to the order Nidovirales, family Arteriviridae =-=(3)-=-, is characterized by reproductive failure in pregnant sows and respiratory disease in piglets (4). PRRSV isolates are classified into two distinct genotypes: the European (EU) and the North American ...
70	Porcine circoviruses: a review - Allan, Ellis - 2000 (Show Context) Citation Context ... surveillance. Natural coinfection of swine with porcine reproductive andrespiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) is common in countries with intensive swine production =-=(1, 2)-=-. Infection with PRRSV, an enveloped positivestrand RNA virus that belongs to the order Nidovirales, family Arteriviridae (3), is characterized by reproductive failure in pregnant sows and respiratory...
69	A very small porcine virus with a circular single-stranded DNA. Nature 295:64–66 - Tischer, Gelderblom, et al. - 1982 (Show Context) Citation Context ...notypes: the European (EU) and the North American (NA) genotypes (5). PCV2, responsible for considerable economic loss in the swine industry worldwide (6), is a nonenveloped single-stranded DNA virus =-=(7)-=-. Infection with PCV2 has been associated with the postweaning multisystemic wasting syndrome (PMWS) (8), where typical clinical signs include weight loss, respiratory distress, and jaundice, as well ...
66	Multiplex PCR: critical parameters and step-by-step protocol. - Henegariu, Heerema, et al. - 1997 (Show Context) Citation Context ...of oligonucleotides in the same reaction tube is a possible interaction of those molecules with each other, resulting in inhibition of the amplification reactions and a subsequent reduced sensitivity =-=(38, 48, 49)-=-. In our assays, despite the minor decrease in sensitivity, 1,000 genome copies per reaction or less were reliably detected by each of the assays included in the multiplex RT-qPCR. With the exception ...
35	Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine - Allan, Kennedy, et al. - 1999 (Show Context) Citation Context ... economic loss in the swine industry worldwide (6), is a nonenveloped single-stranded DNA virus (7). Infection with PCV2 has been associated with the postweaning multisystemic wasting syndrome (PMWS) =-=(8)-=-, where typical clinical signs include weight loss, respiratory distress, and jaundice, as well as pathological findings of interstitial pneumonia, generalized, enlarged lymph nodes, hepatitis, and ne...
32	Postweaning multisystemic wasting syndrome (PMWS) in pigs. A review. - Segales, Domingo - 2002
26	Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Vet - AS, Nielsen, et al. - 2002
25	A universal heterologous internal control system for duplex realtimeRT-PCR assays used in a detection system for pestiviruses,” 12 - Hoffmann, Depner, et al. - 2006 (Show Context) Citation Context ...ations (Table 2). The analytical sensitivity of the complete multiplex RT-qPCR system was determined using series of 10-fold dilutions of the individual positive standards (diluted in RNA safe buffer =-=[33]-=-) in three replicates per run on three different days. RNA extracted from porcine EDTA-blood was included in the master mixture to test for the influence of porcine DNA and RNA. In the multiplex Multi...
24	Porcine reproductive and respiratory syndrome. Vet Pathol 35:1–20 - KD - 1998 (Show Context) Citation Context ...SV, an enveloped positivestrand RNA virus that belongs to the order Nidovirales, family Arteriviridae (3), is characterized by reproductive failure in pregnant sows and respiratory disease in piglets =-=(4)-=-. PRRSV isolates are classified into two distinct genotypes: the European (EU) and the North American (NA) genotypes (5). PCV2, responsible for considerable economic loss in the swine industry worldwi...
21	Porcine circovirus diseases. Anim. Health Res. Rev - Segalés, GM, et al. - 2005 (Show Context) Citation Context ...RRSV isolates are classified into two distinct genotypes: the European (EU) and the North American (NA) genotypes (5). PCV2, responsible for considerable economic loss in the swine industry worldwide =-=(6)-=-, is a nonenveloped single-stranded DNA virus (7). Infection with PCV2 has been associated with the postweaning multisystemic wasting syndrome (PMWS) (8), where typical clinical signs include weight l...
16	AE, Hengartner CJ. 2005. Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev 69:462–500 - LE, Reynolds (Show Context) Citation Context ... fever (CSF), andAujeszky’s disease. Pseudorabies or Aujeszky’s disease, caused by Suid herpesvirus 1 (SuHV1), a member of the genus Varicellovirus, subfamily Alphaherpesvirinae, familyHerpesviridae, =-=(14)-=-, is a notifiable disease in swineof great economic importance.Among thewide rangeof hosts, including nearly all mammals except humans and higher primates, only members of the Suidae family are able t...
16	Ashshi AM, Cooper RJ, Klapper PE. 2000. Multiplex PCR: optimization and application in diagnostic virology - EM (Show Context) Citation Context ...of oligonucleotides in the same reaction tube is a possible interaction of those molecules with each other, resulting in inhibition of the amplification reactions and a subsequent reduced sensitivity =-=(38, 48, 49)-=-. In our assays, despite the minor decrease in sensitivity, 1,000 genome copies per reaction or less were reliably detected by each of the assays included in the multiplex RT-qPCR. With the exception ...
14	TheMIQE guidelines:minimum information for publication of quantitative real-time PCR experiments. Clin. Chem - SA, Benes, et al. - 2009 (Show Context) Citation Context ...h, 1.00E01 copies of the PCV2, ASFV, SuHV-1, and FMDV standards, 1.00E02 copies of CSFV, and 1.00E03 PRRSV-EU and -NA copies per reaction were reliably detected. The mean quantification cycle (Cq) =-=(34)-=- value of the IC was 32.6, with a coefficient of variation of 0.1 (Fig. 1). Finally, to exclude unspecific reactions, nucleic acids extracted from sera, pools of sera, EDTA-blood samples, nasal swabs,...
13	Multiplex PCR: advantages, development, and applications - MC, RA - 1994 (Show Context) Citation Context ...ex PCR systems, several infectious agents can be detected and differentiated simultaneously in a single reaction, reducing costs and efforts as well as the amount of sample material and time required =-=(37, 38)-=-. A variety of conventional multiplex PCR assays for the detection and differentiation of different swine viruses based on amplicon size have been developed during recent years (39–46). However, qPCR ...
12	De Clercq K (2007) Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments - JF, Sailleau, et al. (Show Context) Citation Context ...iagnostics Deutschland GmbH, Mannheim, Germany) according to the manufacturer’s recommendations. Primers, probes, and real-time PCR. The PCV2-specific (27), CSFVspecific (28), and beta-actin-specific =-=(29)-=- real-time quantitative PCR (qPCR) assays have been described previously. PRRSV genome detection was performed as a combination of one assay specific for the EU genotype and another assay detecting NA...
11	Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. - CJ, MP, et al. - 1999 (Show Context) Citation Context ...zed by reproductive failure in pregnant sows and respiratory disease in piglets (4). PRRSV isolates are classified into two distinct genotypes: the European (EU) and the North American (NA) genotypes =-=(5)-=-. PCV2, responsible for considerable economic loss in the swine industry worldwide (6), is a nonenveloped single-stranded DNA virus (7). Infection with PCV2 has been associated with the postweaning mu...
11	Floegel-Niesmann G, Greiser-Wilke I. 2003. Clinical signs and epidemiology of classical swine fever: a review of new knowledge - Moennig (Show Context) Citation Context ...nical or postmortem examination (19). Clinical symptoms vary from sudden death, fever, and hemorrhages of the skin and internal organs to respiratory signs, stunting of growth, anorexia, and lethargy =-=(20, 21)-=-. Similar symptoms were caused by a highly pathogenic strain of PRRSV (HP-PRRSV) since its emergence 2006 in China (22) and subsequent spread to other Asian countries (23, 24). For the effective contr...
11	Siafakas N, Moncany M. 2002. Multiplex polymerase chain reaction: a practical approach - Markoulatos (Show Context) Citation Context ...ex PCR systems, several infectious agents can be detected and differentiated simultaneously in a single reaction, reducing costs and efforts as well as the amount of sample material and time required =-=(37, 38)-=-. A variety of conventional multiplex PCR assays for the detection and differentiation of different swine viruses based on amplicon size have been developed during recent years (39–46). However, qPCR ...
10	Porcine respiratory and reproductive syndrome virus variants, Vietnam and - Feng, Zhao, et al. - 2008 (Show Context) Citation Context ..., anorexia, and lethargy (20, 21). Similar symptoms were caused by a highly pathogenic strain of PRRSV (HP-PRRSV) since its emergence 2006 in China (22) and subsequent spread to other Asian countries =-=(23, 24)-=-. For the effective control and eradication of foot-and-mouthdisease (FMD), a highly contagious disease of cloven-hoofed animals with a great economic impact (25), a rapid diagnosis is crucial. The ca...
10	Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay." - Zsak, Borca - 2005 (Show Context) Citation Context ... was performed as a combination of one assay specific for the EU genotype and another assay detecting NA genotype-specific sequences (30). The ASFV-specific qPCR assay developed by Zsak and coworkers =-=(31)-=- was slightly modified. Instead of the previously described labeling with 5=-6-carboxyfluorescein (FAM) and a 3= minor grove binder nonfluorescent quencher, a Texas Red-labeled locked nucleic acid (LN...
7	Porcine reproductive and respiratory syndrome virus (PRRSV) infection status in pigs naturally affected with post-weaning multisystemic wasting syndrome (PMWS - Segales, Calsamiglia, et al. - 2002 (Show Context) Citation Context ... surveillance. Natural coinfection of swine with porcine reproductive andrespiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) is common in countries with intensive swine production =-=(1, 2)-=-. Infection with PRRSV, an enveloped positivestrand RNA virus that belongs to the order Nidovirales, family Arteriviridae (3), is characterized by reproductive failure in pregnant sows and respiratory...
5	Molecular diagnosis of viral diseases, present trends and future aspects: a view from the OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for Diagnosis of Viral Diseases in Veterinary Medicine. Vaccine 25:5444–5452 - Belak (Show Context) Citation Context ...e geographic areas, which thereby affects animal populations of high density, especially after sudden reemergence in free countries, the rapid diagnosis of the causative agent is of utmost importance =-=(36)-=-. By using multiplex PCR systems, several infectious agents can be detected and differentiated simultaneously in a single reaction, reducing costs and efforts as well as the amount of sample material ...
5	A highly sensitive and specific gel-based multiplex RT-PCR assay for the simultaneous and differential diagnosis of African swine fever and classical swine fever in clinical samples. Vet. Res - Aguero, Fernandez, et al. - 2004
5	Bone marrow depletion with haemorrhagic diathesis in calves in Germany: characterization of the disease and preliminary investigations on its aetiology. Berl.Munch. Tierarztl.Wochenschr - EC, MY, et al. - 2010 (Show Context) Citation Context ...nd in cattle previously (50–52), though this observation was not confirmed by other authors (53, 54). Recently, PCV2 has been detected inGerman calves affected with hemorrhagic disease syndrome (HDS) =-=(50)-=-. The example shows the versatile use of the novel PCR system, which is not only for porcine samplematerials. In conclusion, the newly developed RT-qPCR allows the simultaneous detection of PRRSV and ...
4	Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical findings - JCS - 1996
4	Swine pseudorabies: abortion, clinical disease, and lesions in pregnant gilts Infected with pseudorabies virus (Aujeszky's disease - Kluge, s, et al. - 1974 (Show Context) Citation Context ...eof hosts, including nearly all mammals except humans and higher primates, only members of the Suidae family are able to survive a productive infection and as a consequence serve as a virus reservoir =-=(15)-=-. Clinical signs range from central nervous system disorders and death in young piglets to respiratory disease and reproductive failure in older pigs (16). CSF, caused by classical swine fever virus (...
4	GF. 2007. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS inChina andmolecular dissectionof the unique hallmark. PLoS One 2:e526 - Tian, Yu, et al. (Show Context) Citation Context ...rnal organs to respiratory signs, stunting of growth, anorexia, and lethargy (20, 21). Similar symptoms were caused by a highly pathogenic strain of PRRSV (HP-PRRSV) since its emergence 2006 in China =-=(22)-=- and subsequent spread to other Asian countries (23, 24). For the effective control and eradication of foot-and-mouthdisease (FMD), a highly contagious disease of cloven-hoofed animals with a great ec...
4	A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, andporcine reproductive and respiratory syndrome virus in clinical specimens. Virus Genes 38:392–397 - Yue, Cui, et al. - 2009
3	Family Flaviviridae, p 979–996 - Thiel, MS, et al. - 2005 (Show Context) Citation Context ...to respiratory disease and reproductive failure in older pigs (16). CSF, caused by classical swine fever virus (CSFV), a positivesense single-stranded RNA virus, genus Pestivirus, family Flaviviridae =-=(17)-=-, and African swine fever (ASF), caused by the large enveloped, double-stranded DNA African swine fever virus (ASFV), assigned to be the only member of the genus Asfivirus, familyAsfarviridae (18), ca...
3	African swine fever. Adv. Virus Res - CA - 1988 (Show Context) Citation Context ...nical or postmortem examination (19). Clinical symptoms vary from sudden death, fever, and hemorrhages of the skin and internal organs to respiratory signs, stunting of growth, anorexia, and lethargy =-=(20, 21)-=-. Similar symptoms were caused by a highly pathogenic strain of PRRSV (HP-PRRSV) since its emergence 2006 in China (22) and subsequent spread to other Asian countries (23, 24). For the effective contr...
2	Gene specific assay to differentiate strains of pseudorabies virus. Vet. Microbiol - Hasebe, JG, et al. - 1993 (Show Context) Citation Context ...ll; each of them tested positive by the SuHV-1 assay included in the multiplex PCR (see Table S1, sample ID 230 and 238 to 255) and confirmed by the conventional PCR system described by Hasebe et al. =-=(32)-=- (data not shown). The sensitivity of each assay in the multiplex approach was evaluated in comparison to the corresponding single-target system using 10-fold dilutions of genomes of appropriate cultu...
2	Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections - Ogawa, Taira, et al. - 2009
2	Soji OB, Alam MM, Kunz TH, Zaidi SZ, Delwart E. 2011. Possible cross-species transmission of circoviruses and cycloviruses among farm animals - Li, Shan
2	Evidence for circovirus in cattle with respiratory disease and from aborted bovine fetuses - GP, AL, et al. - 1999
1	de Jong MF, Boersma WJ, Elbers AR. 2004. Excessive porcine circovirus type 2 antibody titres may trigger the development of porcine dermatitis and nephropathy syndrome: a case-control study. Vet. Microbiol - GJ, Stockhofe-Zurwieden (Show Context) Citation Context ...f interstitial pneumonia, generalized, enlarged lymph nodes, hepatitis, and nephritis (9–11). Additionally, PCV2 was suspected to be associated with porcine dermatitis and nephropathy syndrome (PDNS) =-=(12)-=- and reproductive failure (13). The list of differential diagnosis for PMWS includes the respiratory form of PRRS, classical swine fever (CSF), andAujeszky’s disease. Pseudorabies or Aujeszky’s diseas...
1	GM, Pensaert MB. Multiplexed Molecular Detection of Porcine Viruses March 2013 Volume 51 Number 3 jcm.asm.org 943 o n Septem ber 16, 2016 by PENN STATE UNIV http://jcm.asm.org/ D ow nloaded from - RE, HJ, et al. (Show Context) Citation Context ...ralized, enlarged lymph nodes, hepatitis, and nephritis (9–11). Additionally, PCV2 was suspected to be associated with porcine dermatitis and nephropathy syndrome (PDNS) (12) and reproductive failure =-=(13)-=-. The list of differential diagnosis for PMWS includes the respiratory form of PRRS, classical swine fever (CSF), andAujeszky’s disease. Pseudorabies or Aujeszky’s disease, caused by Suid herpesvirus ...
1	Vietnam grapple with ‘rapidly evolving’ pig virus. Science 317:1017 - Normile (Show Context) Citation Context ..., anorexia, and lethargy (20, 21). Similar symptoms were caused by a highly pathogenic strain of PRRSV (HP-PRRSV) since its emergence 2006 in China (22) and subsequent spread to other Asian countries =-=(23, 24)-=-. For the effective control and eradication of foot-and-mouthdisease (FMD), a highly contagious disease of cloven-hoofed animals with a great economic impact (25), a rapid diagnosis is crucial. The ca...
1	Development of a highly sensitive real-time RT-PCR protocol for the detection of classical swine fever virus independent of the 5= untranslated region - Leifer, Blome, et al. (Show Context) Citation Context ... automated extraction (Roche Diagnostics Deutschland GmbH, Mannheim, Germany) according to the manufacturer’s recommendations. Primers, probes, and real-time PCR. The PCV2-specific (27), CSFVspecific =-=(28)-=-, and beta-actin-specific (29) real-time quantitative PCR (qPCR) assays have been described previously. PRRSV genome detection was performed as a combination of one assay specific for the EU genotype ...
1	A multiplex RT-PCR assay for the rapid and differential diagnosis of classical swine fever and other pestivirus infections. Vet. Microbiol - H, LJ, et al. - 2009
1	Sanchez-Vizcaino JM. 2008. Rapid and differential diagnosis of foot-andmouth disease, swine vesicular disease, and vesicular stomatitis by a new multiplex RT-PCR assay - Fernandez, Aguero, et al.
1	use of a multiplex real-time quantitative polymerase chain reaction assay for detection and differentiation of porcine circovirus-2 genotypes 2a and 2b in an epidemiological survey - Development
1	Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs bymultiplex polymerase chain reaction - Jiang, Shang, et al. - 2010
1	AmultiplexRT-PCR for rapid and simultaneous detectionof porcine teschovirus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus in clinical specimens - Liu, Zhao, et al. - 2011
1	MG, Gundry CN, Elenitoba-Johnson KS. 2001. Real-time multiplex PCR assays. Methods 25:430–442 - CT, Herrmann (Show Context) Citation Context ...t al. 942 jcm.asm.org Journal of Clinical Microbiologyso nsSeptem ber 16, 2016 by PENN STATE UNIV http://jcm.asm.org/ D ow nloaded from with different fluorophores permitmultiplexing in a qPCR format =-=(47)-=-, enabling the detection of different target sequences as well as the coamplification of internal controls. Here, an RT-qPCR assay for the simultaneous detection of PRRSV and PCV2, combined with an ea...
1	Lack of antibodies to porcine circovirus type 2 virus in beef and dairy cattle and horses - JA, Konoby, et al. - 2001 (Show Context) Citation Context ...gle-target assay and in the multiplex system. Interestingly, the presence of PCV2-like isolates has been found in cattle previously (50–52), though this observation was not confirmed by other authors =-=(53, 54)-=-. Recently, PCV2 has been detected inGerman calves affected with hemorrhagic disease syndrome (HDS) (50). The example shows the versatile use of the novel PCR system, which is not only for porcine sam...