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... [2]. DNA microarrays, cell microarrays, and tissue microarrays have allowed for cheaper and faster analysis crucial to the genomic era [15]. The tissue microarray, invented by Kononen et al. in 1998 =-=[7]-=-, is one of the most useful of these high throughput techniques. It has applications in both research and clinical settings, for in situ gene expression and immunohistochemical and FISH analysis [11]....

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...f methanol, formaldehyde and acetic acid (85:10:5 by volume) for forty five minutes, treated with acid hydrolysis (5 N HCl) at 26◦C for one hour and stained using a Feulgen–Thionin stain for one hour =-=[6,14]-=-. Image acquisition was performed using the Oncometric Cyto-Savant automated quantitative image cytometer (Department of Cancer Imaging, British Columbia, Cancer Agency, Vancouver, Canada) [11, 13]. S...

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... that characterize microarray techniques, to be applied to cell suspension samples, be they clinical material or cell lines. While there has been some attempt to create TMA-like arrays for cell lines =-=[1,2,16]-=-, the CMA offers many advantages: it is simple, fast, and easily reproducible (Figs 3–5). Despite differences in cell count, the variability of the quantitative analysis of all three deposition method...

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...hour [6,14]. Image acquisition was performed using the Oncometric Cyto-Savant automated quantitative image cytometer (Department of Cancer Imaging, British Columbia, Cancer Agency, Vancouver, Canada) =-=[11, 13]-=-. Slides were exhaustively scanned and the images of each nuclei from all of the 60 spots were stored in the computer memory. Approximately 110 features were calculated for each nuclear image by the i...

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...f methanol, formaldehyde and acetic acid (85:10:5 by volume) for forty five minutes, treated with acid hydrolysis (5 N HCl) at 26◦C for one hour and stained using a Feulgen–Thionin stain for one hour =-=[6,14]-=-. Image acquisition was performed using the Oncometric Cyto-Savant automated quantitative image cytometer (Department of Cancer Imaging, British Columbia, Cancer Agency, Vancouver, Canada) [11, 13]. S...

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...of biological samples from gene expression [4] to glycan binding [2]. DNA microarrays, cell microarrays, and tissue microarrays have allowed for cheaper and faster analysis crucial to the genomic era =-=[15]-=-. The tissue microarray, invented by Kononen et al. in 1998 [7], is one of the most useful of these high throughput techniques. It has applications in both research and clinical settings, for in situ ...

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...ng method is presented in (Fig. 11). MIB-1 immunostaining on cytological specimens is not commonly used, although it would be very valuable for the assessment of tumour proliferation before treatment =-=[3,12]-=-. To evaluate the use of CMA with immunohistochemical techniques we stained all cell line CMAs for antigens MIB-1 using a DakoCytomation Ki-67 Monoclonal Mouse, Clone MIB-1 according to laboratory sta...

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...8 [7], is one of the most useful of these high throughput techniques. It has applications in both research and clinical settings, for in situ gene expression and immunohistochemical and FISH analysis =-=[11]-=-. Constructed by placing an array of paraffin embedded tissue cores into a recipient block, tissue microarrays (TMA) can hold up to several hundred tissue samples per slide [15]. The TMA does have dra...

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... that characterize microarray techniques, to be applied to cell suspension samples, be they clinical material or cell lines. While there has been some attempt to create TMA-like arrays for cell lines =-=[1,2,16]-=-, the CMA offers many advantages: it is simple, fast, and easily reproducible (Figs 3–5). Despite differences in cell count, the variability of the quantitative analysis of all three deposition method...

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...lar markers 1. Introduction Over the past decade and a half a number of high throughput array techniques have revolutionized the study of biological samples from gene expression [4] to glycan binding =-=[2]-=-. DNA microarrays, cell microarrays, and tissue microarrays have allowed for cheaper and faster analysis crucial to the genomic era [15]. The tissue microarray, invented by Kononen et al. in 1998 [7],...

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...hput screening, molecular markers 1. Introduction Over the past decade and a half a number of high throughput array techniques have revolutionized the study of biological samples from gene expression =-=[4]-=- to glycan binding [2]. DNA microarrays, cell microarrays, and tissue microarrays have allowed for cheaper and faster analysis crucial to the genomic era [15]. The tissue microarray, invented by Konon...

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...that the volume of sample deposited per spot was also consistent throughout each depositing event accounting for uniform cell count results (Fig. 3). Using the integrated optical density of a nucleus =-=[4,5]-=- the DNA amount of each cell was measured, and from that the variation and mean DNA value per cell was calculated per spot; shown in Fig. 5. The nuclear area of cells (the number of connected pixels f...

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...ed to any studies done. Our fully automated technology allows for rapid testing of new biological and molecular markers which can be used in conjunction with current cervical cancer screening methods =-=[3,8]-=-. As the array dimensions and spot number are controlled merely by altering the pattern of micropipettes, the CMA is very flexible and can be designed to meet diverse experimental applications. We hav...

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...amples per slide [15]. The TMA does have drawbacks, as it is labour intensive and requires considerable skill to produce error-free recipient blocks. Commercial TMA building instruments are expensive =-=[10]-=- and the technique is not applicable to cell suspension samples, whether for research (cell lines), or pathological diagnosis (sputum, blood, cervical, etc.) *Corresponding author: Jagoda Korbelik, BS...

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...ng method is presented in (Fig. 11). MIB-1 immunostaining on cytological specimens is not commonly used, although it would be very valuable for the assessment of tumour proliferation before treatment =-=[3,12]-=-. To evaluate the use of CMA with immunohistochemical techniques we stained all cell line CMAs for antigens MIB-1 using a DakoCytomation Ki-67 Monoclonal Mouse, Clone MIB-1 according to laboratory sta...