### Citations

61 |
Micropipette aspiration of living cells
- Hochmuth
(Show Context)
Citation Context ...91; Trickey et al., 2000). Among various single cell probing methods, micropipette aspiration (MA) has been used extensively to measure the viscoelastic properties of several cell types (reviewed in (=-=Hochmuth, 2000-=-)) and cellular organelles (Guilak et al., 2000; Rowat et al., 2006; Vaziri and Mofrad, 2007). To estimate conditions, and material and geometric nonlinearities, which putatively lead to more accurate... |

44 | An Application of the Micropipette Technique to the Measurement of the Mechanical Properties of Cultured Bovine Aortic Endothelial Cells,"
- Sato, Levesque, et al.
- 1987
(Show Context)
Citation Context ...timated by FE analyses of MA differ from thatviscoelastic material parameters from MA data, the experimental deformation history is typically fit to a linear viscoelastic half-space analytical model (=-=Sato et al., 1990-=-), in which the cell is an infinitely large half-space undergoing infinitesimal deformation and subjected to instantaneously applied pressure. In reality, however, aspirated cells sample thickness (Al... |

43 |
The application of a homogeneous half-space model in the analysis of endothelial cell micropipette measurements
- Theret, Levesque, et al.
- 1988
(Show Context)
Citation Context ...ilak et al., 1999). In this model the normalized aspiration length of the cell is given as L Rp fpDP 2pG0ð1 a1Þ 1 a1et=t ð1Þ where Fp is a wall function (assumed to be a constant value of 2.1 (=-=Theret et al., 1988-=-)), L the aspiration length, Rp the pipette radius, DP the applied pressure, G0 the instantaneous shear modulus, a1 the dimensionless relaxation modulus, and t the creep time constant. The viscoelasti... |

31 |
Viscoelastic properties of the cell nucleus.
- Guilak, Tedrow, et al.
- 2000
(Show Context)
Citation Context ...ingle cell probing methods, micropipette aspiration (MA) has been used extensively to measure the viscoelastic properties of several cell types (reviewed in (Hochmuth, 2000)) and cellular organelles (=-=Guilak et al., 2000-=-; Rowat et al., 2006; Vaziri and Mofrad, 2007). To estimate conditions, and material and geometric nonlinearities, which putatively lead to more accurate predictions of material parameters than those ... |

19 |
Viscoelastic properties of chondrocytes from normal and osteoarthritic human cartilage
- Trickey
- 1987
(Show Context)
Citation Context ... time-dependent effects due to finite lo rates also impact cell properties estimates (Merryman et al., 20phenotype (Darling et al., 2008) or its transition to a pathological state (Ward et al., 1991; =-=Trickey et al., 2000-=-). Among various single cell probing methods, micropipette aspiration (MA) has been used extensively to measure the viscoelastic properties of several cell types (reviewed in (Hochmuth, 2000)) and cel... |

13 |
Mechanics and deformation of the nucleus in micropipette aspiration experiment. J Biomech 2007;40:2053–62.rmed elastic shells. Thin Walled Struct
- Vaziri, MR
- 2009
(Show Context)
Citation Context ...aspiration (MA) has been used extensively to measure the viscoelastic properties of several cell types (reviewed in (Hochmuth, 2000)) and cellular organelles (Guilak et al., 2000; Rowat et al., 2006; =-=Vaziri and Mofrad, 2007-=-). To estimate conditions, and material and geometric nonlinearities, which putatively lead to more accurate predictions of material parameters than those obtained analytically. FE analyses have shown... |

12 | JH: Mechanical properties of the cell nucleus and the effect of emerin deficiency.
- AC, Lammerding, et al.
- 2006
(Show Context)
Citation Context ...thods, micropipette aspiration (MA) has been used extensively to measure the viscoelastic properties of several cell types (reviewed in (Hochmuth, 2000)) and cellular organelles (Guilak et al., 2000; =-=Rowat et al., 2006-=-; Vaziri and Mofrad, 2007). To estimate conditions, and material and geometric nonlinearities, which putatively lead to more accurate predictions of material parameters than those obtained analyticall... |

11 |
Calcification by valve interstitial cells is regulated by the stiffness of the extracellular matrix. Arteriosclerosis, Thrombosis, and Vascular Biology.
- CYY, Chen, et al.
- 2009
(Show Context)
Citation Context ...rials and methods Detailed methods are described in the Supplemental Material. 2.1. Experimental methods Primary porcine aortic valve interstitial cells (VICs) were isolated by collagenase digestion (=-=Yip et al., 2009-=-) and tested within 3h of isolation. Micropipette aspiration was conducted using a custom-built system similar to those described previously (Trickey et al., 2000). Twenty-five VICs were tested by app... |

9 |
An axisymmetric boundary integral model for assessing elastic cell properties in the micropipette aspiration contact problem.
- 8Haider, Guilak
- 2002
(Show Context)
Citation Context ...the cell (described as Rp/Rc, the ratio of the pipette radius, Rp, to that of the cell, Rc) for all the tested cells, as this is a variable that has been shown to impact material parameter estimates (=-=Haider and Guilak, 2002-=-; Zhou et al., 2005). The effect of loading rate was assessed using the standard half-space model, the superposition half-space model, and the inverse FE method to evaluate the experimental data of in... |

7 |
Applied Regression Analysis, second ed.
- Draper, Smith
- 1981
(Show Context)
Citation Context ...ver the actual loading period. Paired comparisons were made on the goodness of fit and the material parameter estimates. 2.6. Statistics The goodness of fits were evaluated using the R-squared value (=-=Draper and Smith, 1981-=-). Paired and unpaired t-tests were used to determine statistical significance as appropriate. Linear regression was performed using SigmaStat (Systat Software Inc, San Jose, CA). Data are presented a... |

7 |
Cofilin is a marker of myofibroblast differentiation in cells from porcine aortic cardiac valves.
- Pho, Lee, et al.
- 2008
(Show Context)
Citation Context ...wo studies is that we used freshly isolated VICs, whereas Merryman et al. measured the properties of subcultured cells. VICs undergo myofibroblast differentiation on rigid tissue culture polystyrene (=-=Pho et al., 2008-=-; Yip et al., 2009), which is accompanied by a change in their mechanical properties that persists even after detachment from the culture substrate (K. Wyss and C.A. Simmons, unpublished observations)... |

6 | Viscoelastic properties of transformed cells: Role in tumor cell progression and metastasis formation
- Ward, Li
- 1991
(Show Context)
Citation Context ...mulations show that time-dependent effects due to finite lo rates also impact cell properties estimates (Merryman et al., 20phenotype (Darling et al., 2008) or its transition to a pathological state (=-=Ward et al., 1991-=-; Trickey et al., 2000). Among various single cell probing methods, micropipette aspiration (MA) has been used extensively to measure the viscoelastic properties of several cell types (reviewed in (Ho... |

5 |
Inverse finite element characterization of nonlinear hyperelastic membranes
- Kyriacou, Shah, et al.
- 1997
(Show Context)
Citation Context ... in Eq. (2) were determined using a nonlinear leastsquares curve fitting method in Matlab. 2.3. Finite element model In the FE model, the cell was assumed to be a deformable sphere havingmeasurement (=-=Kyriacou et al., 1997-=-; Lei and Szeri, 2007). This procedure was Please cite this article as: Zhao, R., et al., Comparison of analytical an properties by micropipette aspiration. Journal of Biomechanics (200ratios of the i... |

4 |
Viscoelastic Properties of Intervertebral Disc Cells.
- Guilak, Ting-Beall, et al.
- 1999
(Show Context)
Citation Context ...rd half-space and other analytical models A linear viscoelastic half-space model was used to fit the deformation history of a VIC subjected to an instantaneous aspiration pressure (Sato et al., 1990; =-=Guilak et al., 1999-=-). In this model the normalized aspiration length of the cell is given as L Rp fpDP 2pG0ð1 a1Þ 1 a1et=t ð1Þ where Fp is a wall function (assumed to be a constant value of 2.1 (Theret et al., 19... |

4 |
Szeri, “Inverse analysis of constitutive models: biological soft tissues
- Lei, Z
- 2007
(Show Context)
Citation Context ...ined using a nonlinear leastsquares curve fitting method in Matlab. 2.3. Finite element model In the FE model, the cell was assumed to be a deformable sphere havingmeasurement (Kyriacou et al., 1997; =-=Lei and Szeri, 2007-=-). This procedure was Please cite this article as: Zhao, R., et al., Comparison of analytical an properties by micropipette aspiration. Journal of Biomechanics (200ratios of the initial guesses to the... |

4 | Viscoelastic properties of the aortic valve interstitial cell - Merryman, Bieniek, et al. - 2009 |

2 | Analysis user’s manual, version - ABAQUS - 2008 |