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...tant mARF10 driven by its own 5¢ upstream regulatory sequence were analyzed. The phenotypes of mutant seeds and seedlings showed the importance of auxin signaling in the early stages of plant growth. A key role of miR160 and ARF10 in interaction between auxin and ABA during germinative and post-germinative stages was identified. Results Expression of miR160-resistant ARF10 causes developmental defects in leaves, flowers and siliques The ARF10 protein contains a conserved family domain and a B3 domain, which occurs in multiple transcription factors including LEC2 (Stone et al., 2001) and FUS3 (Luerssen et al., 1998; Figure 1a). The miR160 target site (5¢-AGGAAUACAGGGAGCCAGGCA-3¢) in ARF10 mRNA is located at a position corresponding to the amino acid sequence GIQGAR. The miR160 sequence shows high complementarity to the ARF10 sequence, with only two mis-matched bases. The ARF10 gene, including the coding region (2.3 kb) and the 5¢ upstream region (3 kb) (ARF10:ARF10) (Figure 1b), was amplified by PCR, and then fourmutationswere introduced into the miR160 target site by PCR to generate ARF10:mARF10 (Figure 1a). The ARF10 and mARF10 sequences were fused to the green fluorescent protein sequence (ARF10:ARF1...

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... (Harada 2001). Loss‐of‐function of LEC genes partially loses embryo identity, while loss‐of‐function of FUSCA3 and ABI3 yield defective and ABA‐insensitive embryos, respectively (Meinke et al. 1994; =-=Luerßen et al. 1998-=-). In addition, SHORT HYPOCOTYL UNDER BLUE1 (SHB1) is a positive regulator of seed development, which is required for the proper expression of two other transcription factors that affect endosperm dev...

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...additional factors involved and to build an integrated genetic network. In Arabidopsis, ABI3, FUS3, LEC1, and LEC2 are master regulators of seed maturation (Giraudat et al., 1992; Lotan et al., 1998; =-=Luerssen et al., 1998-=-; Stone et al., 2001), and they regulate each other (Kagaya et al., 2005b; To et al., 2006). ABI3, FUS3, and LEC2 are closely related members of a plant-specific B3-domain transcription factor family....

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...seed oil synthesis and ALTERED TRYPTOPHAN REGULATION1 (ATR1) for seed GLS synthesis were analyzed. LEC1 encodes an NFY-B-type or CCAAT-binding factortype transcription factor [30]. LEC2 [31] and FUS3 =-=[32]-=- encode proteins also belong to the plant-specific B3 transcription factor gene family. The three key genes have been identified as controllers of seed maturation in Arabidopsis [33]. Compared to wild...

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...The super-family of B3 transcription factors comprises thosesthat have been described to be involved in seed development and ABA-responsive expression, suchsABI3, LEC2 and FUS3 (Giraudat et al. 1992; =-=Luerssen et al. 1998-=-; Stone et al. 2001). Recently,sRAV1 transcription factor has also been implicated as a cold-responsive factor and a negativesregulatory component of growth development (Hu et al. 2004; Yamasaki et al...

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...INSENSITIVE3 (ABI3), LEAFY COTYLEDON1 (LEC1), and LEC2. ABI3, FUS3, and LEC2 encode related plant-specific transcription factors containing the conserved B3 DNA binding domain (Giraudat et al., 1992; =-=Luerssen et al., 1998-=-; Stone et al., 2001), whereas LEC1 encodes a CBF transcription factor (Lotan et al., 1998). All four abi3, lec1, lec2, and fus3 mutants are severely affected in seed maturation and share some common ...

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...sset to 50, and the minimum width of each motif was set to eightsamino acid residues.sExpression of HvFUS3 in Arabidopsis fus3 loss-of-functionsand wild-type genetic backgroundssCol-0 and fus3-3 gl1 (=-=Luerssen et al., 1998-=-) transgenic plants carrying a fusion of the At2S3 promoter and the coding sequence of thesGFP gene have been previously described by Kroj et al. (2003). ThesP35S:HvFus3 and P35SAtFus3 constructs were...

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...ld in the Zhewan 1 seeds compared with the Zhongwan 6 seeds at 25 DAP. Previous studies have reported that FUS3 functions primarily or exclusively as a transcriptional activator during embryogenesis (=-=Luerssen et al., 1998-=-). These results suggested that FUS3 could be a direct regulator of pea seed filling; however, the mechanism requires further study. When combined, our results indicated that TF gene expression underg...