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15
The regulator of MAT2 (ROM2) protein binds to early maturation promoters and represses pvALF-activated transcription
- Plant Cell
, 1996
"... The regulation of maturation (MAf)- and late embryogenesis (MA)-specific gene expression in dicots involves factors related to AB13, a seed-specific component of the abscisic acid signal transduction pathways from Arabidopsis. In French bean (Phaseolus vulgaris), the ABI3-like factor, PvALF, activat ..."
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Cited by 11 (2 self)
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The regulation of maturation (MAf)- and late embryogenesis (MA)-specific gene expression in dicots involves factors related to AB13, a seed-specific component of the abscisic acid signal transduction pathways from Arabidopsis. In French bean (Phaseolus vulgaris), the ABI3-like factor, PvALF, activates transcription from MAT promoters of phytohemagglutinin (DLEC2) and P-phaseolin (PHSB) genes. We describe theIegulatorgf MAT2 (ROM2) as a basic leucine zipper (bZIP) DNA binding protein that recognizes motifs with symmetric (ACGT) and asymmetric (ACCT) core elements present in both MAT promoters. ROM2 antagonizes trans-activation of the DLEC2 promoter by PvALF in transient expression assays. Repression was abolished by mutations that prevented binding of ROM2 to the DLEC2 seed enhancer region. Momover, a hybrid protein composed of a PvALF activation domain and the DNA binding and dimerization domain of ROM2 activated gene expression, indicating that ROM2 recognizes the DLEC2 enhancer in vivo; consequently, ROM2 functions as a DNA binding site-dependent repressor. Supershift analysis of nuclear proteins, using a ROM2-specific antibody, revealed an increase in ROM2 DNA binding activity during seed desiccation. A corresponding increase in ROM2 mRNA coincided with the period when DLEC2 mRNA levels declined in embryos. These results demonstrate developmental regulation of the ROM2 repressor and point to a role for this factor in silencing DLEC2 transcription during late embryogenesis.
Mini-Review Transcriptional Regulation of Storage Protein Synthesis During Dicotyledon Seed Filling
"... Seeds represent a major source of nutrients for human and animal livestock diets. The nutritive value of seeds is largely due to storage products which accumulate during a key phase of seed development, seed filling. In recent years, our understanding of the mechanisms regulating seed filling has ad ..."
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Seeds represent a major source of nutrients for human and animal livestock diets. The nutritive value of seeds is largely due to storage products which accumulate during a key phase of seed development, seed filling. In recent years, our understanding of the mechanisms regulating seed filling has advanced significantly due to the diversity of experimental approaches used. This review summarizes recent findings related to transcription factors that regulate seed storage protein accumulation. A framework for the regulation of storage protein synthesis is established which incorporates the events before, during and after seed storage protein synthesis. The transcriptional control of storage protein synthesis is accompanied by physiological and environmental controls, notably through the action of plant hormones and other intermediary metabolites. Finally, recent post-genomics ana-lyses on different model plants have established the existence of a conserved seed filling process involving the master regulators (LEC1, LEC2, ABI3 and FUS3) but also revealed certain differences in fine regulation between plant families.
unknown title
"... The transition from embryo development to germination is a highly complex developmental process (Bewley, 1997; Holdsworth et al., 1999; McCarty, 1995). At some point during seed development, the embryo enters a period of dormancy, ..."
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The transition from embryo development to germination is a highly complex developmental process (Bewley, 1997; Holdsworth et al., 1999; McCarty, 1995). At some point during seed development, the embryo enters a period of dormancy,
SEE PROFILE
, 2000
"... All in-text references underlined in blue are linked to publications on ResearchGate, letting you access and read them immediately. ..."
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All in-text references underlined in blue are linked to publications on ResearchGate, letting you access and read them immediately.
from the male gametophyte perspective
, 2005
"... Androgenic switch: an example of plant embryogenesis ..."
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A Network of Local and Redundant Gene Regulation Governs Arabidopsis Seed Maturation
"... have been edited and the authors have corrected proofs, but before the final, complete issue is published online. Early posting of articles reduces normal time to publication by several weeks. ..."
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have been edited and the authors have corrected proofs, but before the final, complete issue is published online. Early posting of articles reduces normal time to publication by several weeks.
REVIEW ARTICLE Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective
, 2005
"... Embryogenesis in plants is a unique process in the sense that it can be initiated from a wide range of cells other than the zygote. Upon stress, microspores or young pollen grains can be switched from their normal pollen development towards an embryogenic pathway, a process called androgenesis. Andr ..."
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Embryogenesis in plants is a unique process in the sense that it can be initiated from a wide range of cells other than the zygote. Upon stress, microspores or young pollen grains can be switched from their normal pollen development towards an embryogenic pathway, a process called androgenesis. Androgenesis represents an important tool for research in plant genetics and breeding, since androgenic embryos can germinate into completely homozygous, double haploid plants. From a developmental point of view, androgenesis is a rewarding system for understanding the process of embryo formation from single, haploid microspores. Androgenic development can be divided into three main characteristic phases: acquisition of
Summary
"... The phytohormone abscisic acid (ABA) triggers an oscillation in the cytosolic Ca2 � concentration, which is then perceived by unknown Ca2 � binding proteins to initiate a series of signaling cascades that control many ..."
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The phytohormone abscisic acid (ABA) triggers an oscillation in the cytosolic Ca2 � concentration, which is then perceived by unknown Ca2 � binding proteins to initiate a series of signaling cascades that control many
A Network of Local and Redundant Gene Regulation Governs Arabidopsis Seed Maturation
"... [LEC1], and LEC2) control most aspects of seed maturation, such as accumulation of storage compounds, cotyledon identity, acquisition of desiccation tolerance, and dormancy. The molecular basis for complex genetic interactions among these regulators is poorly understood. By analyzing ABI3 and FUS3 e ..."
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[LEC1], and LEC2) control most aspects of seed maturation, such as accumulation of storage compounds, cotyledon identity, acquisition of desiccation tolerance, and dormancy. The molecular basis for complex genetic interactions among these regulators is poorly understood. By analyzing ABI3 and FUS3 expression in various single, double, and triple maturation mutants, we have identified multiple regulatory links among all four genes. We found that one of the major roles of LEC2 was to upregulate FUS3 and ABI3. The lec2 mutation is responsible for a dramatic decrease in ABI3 and FUS3 expression, and most lec2 phenotypes can be rescued by ABI3 or FUS3 constitutive expression. In addition, ABI3 and FUS3 positively regulate themselves and each other, thereby forming feedback loops essential for their sustained and uniform expression in the embryo. Finally, LEC1 also positively regulates ABI3 and FUS3 in the cotyledons. Most of the genetic controls discovered were found to be local and redundant, explaining why they had previously been overlooked. This works establishes a genetic framework for seed maturation, organizing the key regulators of this process into a hierarchical
unknown title
"... Germination in higher plants is under the control of a combination of internal and environmental factors. The conditions affecting germination include temperature, moisture and the availability of oxygen. In many cases, ..."
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Germination in higher plants is under the control of a combination of internal and environmental factors. The conditions affecting germination include temperature, moisture and the availability of oxygen. In many cases,
