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Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus
- Microbiol. Mol. Biol. Rev. 2005
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Cited by 45 (5 self)
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These include: This article cites 370 articles, 187 of which can be accessed free at:
Severe Acute Respiratory Syndrome Coronavirus nsp1 Suppresses Host Gene Expression, Including That of Type I Interferon, in Infected Cells �
, 2007
"... These include: This article cites 57 articles, 38 of which can be accessed free at: ..."
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Cited by 38 (3 self)
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These include: This article cites 57 articles, 38 of which can be accessed free at:
Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity
- J
, 2004
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Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex
- J. Virol. 2006
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The papain-like protease from the severe acute respiratory syndrome coronavirus is a deubiquitinating enzyme
- J
, 2005
"... This article cites 44 articles, 18 of which can be accessed free ..."
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Cited by 31 (0 self)
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This article cites 44 articles, 18 of which can be accessed free
A novel severe acute respiratory syndrome coronavirus protein, U274, is transported to the cell surface and undergoes endocytosis
- J. Virol
, 2004
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Emerging respiratory viruses: challenges and vaccine
, 2006
"... This article cites 240 articles, 106 of which can be accessed ..."
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Cited by 16 (0 self)
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This article cites 240 articles, 106 of which can be accessed
High Fidelity of Murine Hepatitis Virus Replication Is Decreased in
, 2007
"... Replication fidelity of RNA virus genomes is constrained by the opposing necessities of generating sufficient diversity for adaptation and maintaining genetic stability, but it is unclear how the largest viral RNA genomes have evolved and are maintained under these constraints. A coronavirus (CoV) n ..."
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Cited by 13 (0 self)
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Replication fidelity of RNA virus genomes is constrained by the opposing necessities of generating sufficient diversity for adaptation and maintaining genetic stability, but it is unclear how the largest viral RNA genomes have evolved and are maintained under these constraints. A coronavirus (CoV) nonstructural protein, nsp14, contains conserved active-site motifs of cellular exonucleases, including DNA proofreading enzymes, and the severe acute respiratory syndrome CoV (SARS-CoV) nsp14 has 3�-to-5 � exoribonuclease (ExoN) activity in vitro. Here, we show that nsp14 ExoN remarkably increases replication fidelity of the CoV murine hepatitis virus (MHV). Replacement of conserved MHV ExoN active-site residues with alanines resulted in viable mutant viruses with growth and RNA synthesis defects that during passage accumulated 15-fold more mutations than wild-type virus without changes in growth fitness. The estimated mutation rate for ExoN mutants was similar to that reported for other RNA viruses, whereas that of wild-type MHV was less than the established rates for RNA viruses in general, suggesting that CoVs with intact ExoN replicate with unusually high fidelity. Our results indicate that nsp14 ExoN plays a critical role in prevention or repair of nucleotide incorporation errors during genome replication. The established mutants are unique tools to test the hypothesis that high replication fidelity is required for the evolution and stability of large RNA genomes. Viruses possessing RNA genomes have been proposed to
Structural genomics of the severe acute respiratory syndrome coronavirus: nuclear magnetic resonance structure of the protein nsP7
- J. Virol
, 2005
"... Here, we report the three-dimensional structure of severe acute respiratory syndrome coronavirus (SARS-CoV) nsP7, a component of the SARS-CoV replicase polyprotein. The coronavirus replicase carries out regulatory tasks involved in the maintenance, transcription, and replication of the coronavirus g ..."
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Here, we report the three-dimensional structure of severe acute respiratory syndrome coronavirus (SARS-CoV) nsP7, a component of the SARS-CoV replicase polyprotein. The coronavirus replicase carries out regulatory tasks involved in the maintenance, transcription, and replication of the coronavirus genome. nsP7 was found to assume a compact architecture in solution, which is comprised primarily of helical secondary structures. Three helices (2 to 4) form a flat up-down-up antiparallel -helix sheet. The N-terminal segment of residues 1 to 22, containing two turns of -helix and one turn of 310-helix, is packed across the surface of 2 and 3 in the helix sheet, with the -helical region oriented at a 60 ° angle relative to 2 and 3. The surface charge distribution is pronouncedly asymmetrical, with the flat surface of the helical sheet showing a large negatively charged region adjacent to a large hydrophobic patch and the opposite side containing a positively charged groove that extends along the helix 1. Each of these three areas is thus implicated as a potential site for protein-protein interactions. The severe acute respiratory syndrome coronavirus (SARS-CoV) most closely resembles the group II coronaviruses, which infect mice, rats, pigs, and humans (34). Upon viral entry, the 30-kb SARS-CoV genome is translated to produce a pre-
Stem-loop IV in the 5 untranslated region is a cis-acting element in bovine coronavirus defective interfering RNA replication
- J
, 2005
"... This article cites 42 articles, 32 of which can be accessed free ..."
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Cited by 8 (4 self)
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This article cites 42 articles, 32 of which can be accessed free
