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The genome organization of the Nidovirales: similarities and differences between arteri-, toro-, and coronaviruses
- Semin Virol
, 1997
"... Viruses in the families Arteriviridae and Coronaviridae have enveloped virions which contain nonseg-mented, positive-stranded RNA, but the constituent genera differ markedly in genetic complexity and virion structure. Nevertheless, there are striking resemblances among the viruses in the organizatio ..."
Abstract
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Cited by 49 (11 self)
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Viruses in the families Arteriviridae and Coronaviridae have enveloped virions which contain nonseg-mented, positive-stranded RNA, but the constituent genera differ markedly in genetic complexity and virion structure. Nevertheless, there are striking resemblances among the viruses in the organization and expression of their genomes, and sequence conservation among the polymerase polyproteins strongly suggests that they have a common ancestry. On this basis, the International Committee on Taxonomy of Viruses recently established a new order, Nidovirales, to contain the two families. Here, the common traits and distinguishing features of the Nidovirales are reviewed. r 1997 Academic Press KEY WORDS: arterivirus; coronavirus; torovirus; polyprotein processing; RNA recombination.
Characterization f murine coronavirus RNA by hybridization with virusspecific cDNA probes
- Journal of General Virology
, 1983
"... Genome RNA of mouse hepatitis virus (MHV) strain A59 has been used as a template to synthesize two virus-specific probes: cDNA~ep, representing the majority of sequences of the genome RNA and cDNAy, representing the 3 ' end of the genome RNA. Molecular hybridization with these cDNAs was used to ..."
Abstract
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Cited by 1 (0 self)
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Genome RNA of mouse hepatitis virus (MHV) strain A59 has been used as a template to synthesize two virus-specific probes: cDNA~ep, representing the majority of sequences of the genome RNA and cDNAy, representing the 3 ' end of the genome RNA. Molecular hybridization with these cDNAs was used to characterize both genome RNA and intracellular virus-specific RNAs. Hybridization of genome RNAs of MHV strains A59, JHM, and MHV-3 with A59 cDNArep showed that, although these three strains exhibit different pathogenicities, they contain closely related nucleotide sequences. Hybridization of intracellular RNA from MHV-infected cells with virus-specific DNA shows that (i) the majority of virus-specific RNA is polyadenylated, (ii) virus-specific ntracellular RNA contains ix subgenomic species of the same polarity as genome RNA and (iii) all subgenomic RNAs contain the same Y sequences as the genome RNA and thus form a nested set of RNAs.
SUMMARY
, 1982
"... The multiplication of murine coronavirus strains A59 or JHM in Sac (-) cells was unaffected by the presence of ~-amanitin at concentrations which inhibited the host cell DNA-dependent RNA polymerase activity. In cells infected with the A59 virus strain, actinomycin D-resistant RNA synthesis could r ..."
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The multiplication of murine coronavirus strains A59 or JHM in Sac (-) cells was unaffected by the presence of ~-amanitin at concentrations which inhibited the host cell DNA-dependent RNA polymerase activity. In cells infected with the A59 virus strain, actinomycin D-resistant RNA synthesis could readily be detected by pulse-labelling with [3H]uridine; this virus-specific RNA synthesis was not induced in the presence of the protein synthesis inhibitor anisomycin. A new RNA-dependent RNA polymerase activity was detected in the large particle fraction of A59 virus-infected cells. Optimal conditions for enzyme activity in vitro were established. Maximum activity occurred 5 h after infection, coincident with the peak of virus-specific RNA synthesis detected by pulse-labelling in vivo.
CONTENT ALERTS
, 2000
"... This article cites 45 articles, 24 of which can be accessed free ..."
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CONTENT ALERTS
, 2004
"... This article cites 49 articles, 29 of which can be accessed free ..."
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CONTENT ALERTS
, 2003
"... This article cites 82 articles, 47 of which can be accessed free ..."
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