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...e bond and it was concluded that p65 encompasses the MHV-A59 ORF1aencoded amino acids 248–832. A slightly different processing pattern has been reported for the JHM strain of MHV (Baker et al., 1989; =-=Gao et al., 1996-=-; Schiller et al., 1998). In this case, cleavage products of 28, 72 and 250 kDa were identified. Pulse–chase experiments revealed that the 72 kDa protein, p72, is further processed to a 65 kDa protein...

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...weights of 65, 50, 240, and 290 kDa in MHV-infected cells (80,81) showed that processing of the N-terminus of POL1a involves multiple cleavage events. p65 is thought to be immediately adjacent to p28 =-=(81,82)-=-. Gao et al. (82) reported that p65 of MHV strain JHM is generated from a p72 precursor, but this precursor has not been observed by others studying MHV strain A59 (81). Kinetic analysis suggests that...

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...ysteine protease superfamily JHCReview: Arterivirus molecular biology (Snijder et al., 1992; Lee et al., 1991; Baker et al., 1993; Bonilla et al., 1995; den Boon et al., 1995a; Snijder et al., 1995; =-=Gao et al., 1996-=-). The variability of the N-terminal half of the ORF1a protein suggests that species-specific, rather than groupspecific, functions have been added to the set of basic functions provided by the ‘core ...

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...ts assigned to these polypeptides. In addition, processing of MHV-JHM pp1a results in production of p72, identi�ed to be a precursor of p65; which is not detected in MHV-A59infected cells (Figure 1B; =-=Gao et al, 1996-=-). Schiller et al (1998) proposed that p72 resulted from cleavage between Gly-247 and Val-248 (at the amino terminus) and between Gly-904 and Val-905 (at the carboxyl terminus; Table 1). The authors f...

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...critical for embedding the replicase complex into cellular membranes. p150 itself is extensively processed by MHV 3CLpro (37). In addition, the region upstream of p150 is processed by the PLP1 domain =-=(2, 3, 5, 6, 8, 16)-=-. Therefore, at least three distinct proteinase activities, PLP1, PLP2, and 3CLpro, are required for the maturation of the MHV replicase complex. Previously, we identified the p150 intermediate as a p...

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...n and a 195-kDa protein in IBV-infected cells (23, 24, 26). Similar enzymatic activities were also identified for the first and second papain-like proteinase domains of human and murine coronaviruses =-=(1, 2, 3, 11, 14, 17, 20, 21, 36, 45)-=-. Processing of the 1a and 1a/1b polyproteins at other positions is mediated by the 3C-like proteinase. This proteinase was shown to recognize several conserved Q-S (G and N) dipeptide bonds, resultin...

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... limited thus far. Only the role of this part of the replicase in proteolytic processing has been firmly established by the identification of multiple protease domains in all nidovirus ORF1a proteins =-=(1, 3, 8, 17, 25, 26, 38, 40, 41, 55)-=-. The results presented in this paper indicate that contributing to the formation of a membrane-associated scaffold for the viral replication complex is an important additional function of the ORF1a p...

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...tein, which is a precursor of p27 and is expected to include both membrane-associated protein (MP-1 and MP-2) domains (45). Furthermore, a 72-kDa precursor to p65 has also been identified for MHV-JHM =-=(20, 45)-=-. The processing of ORF 1b products of MHV-A59 has been studied with in vitro-translated products of viral genomic RNA (14). Five products, p90, p74, p53, p44, and p32, were detected, but the existenc...