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...say, which is a simple, inexpensive and widely employed method for the evaluation of antioxidant capacity [20], is based on the capacity of antioxidants to reduce ferric(III) ions to ferrous(II) ions =-=[31]-=-. The FRAP values of the 56 wild fruits are given in Table 1. As seen from the data in the table, the FRAP values varied from 0.67 ± 0.04 to 143 ± 2.00 μmol Fe(II)/g wet weight with a 213-fold differe...

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...ample. ABTS/TEAC (Trolox ® -equivalent antioxidant capacity) [73,74] and DPPH [75-77] are decolorization assays, whereas in Folin total phenols assay [78,79], FRAP (ferric reducing antioxidant power) =-=[80,81]-=- and CUPRAC (cupric reducing antioxidant capacity) [82,83], there is an increase in absorbance at a prespecified wavelength as the antioxidant reacts with the chromogenic reagent (i.e., in the latter ...

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...ght of herbal material. 3.4. Ferric Reducing Antioxidant Power Assay Measurement of ferric reducing antioxidant power of the herbal extract was carried out based on the procedure of Benzie and Strain =-=[27]-=-. Firstly, sodium acetate buffer (300 mmol/L, pH 3.6), 10 mmol/L TPTZ solution (40 mmol/L HCl as solvent) and 20 mmol/L iron (III) chloride solution were mixed in a volume ratio of 10:1:1 to generate ...

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...say The FRAP assay measures the reducing potential of an antioxidant that reacts with a ferric tripyridyltriazine (Fe 3+ - TPTZ) complex to produce a colored ferrous tripyridyltriazine (Fe 2+ - TPTZ) =-=[19,20]-=-. Generally, the reducing properties are associated with the presence of compounds, which exert their action by breaking the free radical chair through the donation of a hydrogen atom [21,22]. The FRA...

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...e of this thermodynamic property [41].Molecules 2010, 15 6911 2.1.4. Ferric reducing ability of plasma The ferric reducing ability of plasma (FRAP) assay was initially described by Benzie and Strain =-=[42]-=- for measuring reducing power in plasma and subsequently adapted and modified by numerous researchers to measure antioxidant power of botanical extracts in their ability to reduce Fe 3+ to Fe 2+ . The...

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...(1.5 mg/ml) and were centrifuged at 3000 g for 10 min to obtain plasma. Erythrocytes were subjected to hypotonic haemolysis by dilution with distilled water. Plasma samples and erythrocyte lysates were stored at −70 ◦C. For the measurement of 8-isoprostane, blood was collected in plastic tubes treated with the antioxidant butylated hydroxytoluene (final concentration, 1 mmol/l). Assays of oxidative-stress-related parameters FRAP (ferric reducing ability of plasma) Plasma antioxidant status was assessed by measuring its ability to reduce ferric to ferrous iron, with a detection limit 10 µmol/l [28]. The inter-assay and intra-assay CVs (coefficients of variation) for FRAP were 3.0 and 1.0 % respectively. Glutathione GSH and GSSG levels were assayed by fluorimetry in erythrocytes and the GSH/GSSG ratio was calculated as a parameter of intracellular redox status [29]. The interand intra-assay CVs for GSH and GSSG were 3.1 and 4.2 % and 2.7 and 3.5 % respectively MDA (malondialdehyde) Erythrocyte lipid peroxidation was assayed spectrophotometrically at 532 nm by the thiobarbituric acid reaction at pH 3.5, followed by solvent extraction with a mixture of n-butanol/pyridine (15:1, v/v) [30]. ...

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... – ∆A517 of sample)/∆A517 of control] × 100Molecules 2010, 15 429 3.7. Reducing antioxidant power effects Reducing power antioxidant effects were determined following the method by Benzie and Strain =-=[32]-=-. Briefly, FRAP reagent (3 mL), prepared freshly, was mixed with test sample (0.1 mL), or methanol (for the reagent blank). The FRAP reagent contained 10 mM TPTZ solution (2.5 mL) in 40 mM HCl plus 20...

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...nic acid) (ABTS) assay [58], the thiobarbituric acid reactant substances (TBARS) [59], Trolox equivalent antioxidant capacity (TEAC) assay [60], and the ferric reducing antioxidant power (FRAP) assay =-=[61]-=-. Resource Table 2. The antioxidant capacities of the extracts from different parts of grape and its products. TEAC a grape pomace 0.91 g/L (EC50 ) - 0.20 g/L (EC50 ) FRAP DPPH ORAC Ref. - [51] grape ...