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Suitable in vitro medium for studying pollen viability in some of the Iranian hawthorn genotypes
, 2010
"... Hawthorn is one of the most important medicinal plants with more nutrients, medical, ornamental and sanitary properties and used as a main dry tolerant rootstock recently. Commercial part of the hawthorn is its fruits and the most important factors involved in fruit set are pollination, pollen tube ..."
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Hawthorn is one of the most important medicinal plants with more nutrients, medical, ornamental and sanitary properties and used as a main dry tolerant rootstock recently. Commercial part of the hawthorn is its fruits and the most important factors involved in fruit set are pollination, pollen tube growth and fertilization. This study was carried out to investigate pollen germination and tube growth of some EastAzerbaijan, Iran indigenous hawthorn genotypes in different in-vitro media for receiving the suitable medium for pollen germination and identifying favourable genotypes for using in the future breeding and orchard establishment programs. Experiment accomplished based on completely randomized design (CRD) in four repeats. Pollens gathered from anthers and maintained at refrigerator until usage. In-vitro medium has different concentrations of sucrose (5, 10, 15 and 20%), boric acid (0.005, 0.01 and 0.02%) with (1.2%) agar. Pollens were cultured in different media and maintained about 24 h in 22˚C and then, their growth was stopped with raising cholorophorm. Finally, pollen germination percentage and tube growth were measured under light-microscope in 7 randomized selected squares. Results showed that the best in-vitro medium for hawthorn pollen germination and tube growth were composed of 15% sucrose, 0.005 -0.01% boric acid and 1.2% agar and significant differences observed between various media in the studied pollen traits
In vitro POLLEN VIABILITY, GERMINATION AND POLLEN TUBE GROWTH IN SOME
"... Abstract. In this study in vitro pollen viability and germination in intact pollens and pol-len tube growth in germinated pollens of five common pomegranate cultivars grown in Croatia and Bosnia and Herzegovina was investigated first time. Pollen viability varied from 36.73 % (cv. Konjski Zub) to 51 ..."
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Abstract. In this study in vitro pollen viability and germination in intact pollens and pol-len tube growth in germinated pollens of five common pomegranate cultivars grown in Croatia and Bosnia and Herzegovina was investigated first time. Pollen viability varied from 36.73 % (cv. Konjski Zub) to 51.80 % (cv. Barski) in fluorescent diacetat (FDA) test. The average pollen germination percentages were found as the lowest 6.83 % in cv. Kon-jski Zub and the highest 42.51 % in cv Glavas. Among the germination media in general 0.2 agar + 10 % sucrose+5 ppm H3BO3 gave better results to obtain higher pollen germina-tion for all cultivars. In germinated pollens of these five cultivars, significant differences were observed on pollen tube length. Average pollen tube lengths in germinated pollens of five cultivars were measured as minimum 302 ȝm in cv. Konjski Zub and as maximum 344 ȝm in cv. Ciparski. The results showed that there were obvious differences in pollen germinability among cultivars growing under the same environmental conditions and these differences must be considered for orchard establishment to obtain higher yield.
Janković et al. Page 207
, 2014
"... Genotypic specificity of walnut (Juglans regia L.) pollen germination on different germination media ..."
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Genotypic specificity of walnut (Juglans regia L.) pollen germination on different germination media
unknown title
, 2010
"... Pollen viability and in vitro germination of selected Central European species from genus Rosa analysed with different methods ..."
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Pollen viability and in vitro germination of selected Central European species from genus Rosa analysed with different methods
