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A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli
- J Bacteriol
, 2004
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These include: This article cites 39 articles, 21 of which can be accessed free at:
2005. Uropathogenic Escherichia coli flagella aid in efficient urinary tract colonization
"... In the murine model of urinary tract infections (UTI), cystitis by uropathogenic Escherichia coli (UPEC) occurs through an intimate relationship with the bladder superficial umbrella cell entailing cycles of adherence, invasion, intracellular bacterial community (IBC) formation, and dispersal (fluxi ..."
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In the murine model of urinary tract infections (UTI), cystitis by uropathogenic Escherichia coli (UPEC) occurs through an intimate relationship with the bladder superficial umbrella cell entailing cycles of adherence, invasion, intracellular bacterial community (IBC) formation, and dispersal (fluxing) from the intracellular environment. IBC dispersal is a key step that results in the spread of bacteria over the epithelial surface to initiate additional rounds of IBC formation. We investigated the role of flagella in mediating adherence and motility during UTI, hypothesizing that the dispersion of the IBC would be incomplete in the absence of motility, thus interrupting the IBC pathway and attenuating the infection. Using gfp reporter fusions, the expression of the flagellar class I flhDC and class III fliC genes was monitored to track key points of regulation throughout the pathogenic cascade. In vitro, growth under conditions promoting motility resulted in the robust expression of both fusions. In contrast, only the class I fusion produced significant expression throughout early stages of IBC development including the dispersion stage. Thus, unlike in vitro modeling of motility, the regulatory cascade appeared incomplete in vivo. Throughout IBC formation, nonmotile �fliC mutants achieved the same number of IBCs as the wild-type (wt) strain, demonstrating that flagella are neither essential nor required for first- or second-generation IBC formation. However, in competition experiments between wt and �fliC strains, the wt was shown to have a fitness advantage in persisting throughout the urinary tract for
Role of the extracytoplasmic function protein family sigma factor RpoE in metal resistance of Escherichia coli
- J
, 2005
"... This article cites 61 articles, 41 of which can be accessed free ..."
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This article cites 61 articles, 41 of which can be accessed free
The Salmonella SPI1 Type Three Secretion System Responds to
, 2007
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CfaD-Dependent Expression of a Novel Extracytoplasmic Protein from Enterotoxigenic Escherichia coli �
, 2007
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This article cites 34 articles, 21 of which can be accessed free
WW: The htx and ptx operons of Pseudomonas stutzeri WM88 are new members of the pho regulon
- J Bacteriol
"... This article cites 38 articles, 20 of which can be accessed free ..."
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This article cites 38 articles, 20 of which can be accessed free
Repression of the inner membrane lipoprotein NlpA by Rns in enterotoxigenic Escherichia coli
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, 2007
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This article cites 33 articles, 21 of which can be accessed free
Expression Levels Influence Ribosomal Frameshifting at the Tandem
, 2004
"... The rare codons AGG and AGA comprise 2 % and 4%, respectively, of the arginine codons of Escherichia coli K-12, and their cognate tRNAs are sparse. At tandem occurrences of either rare codon, the paucity of cognate aminoacyl tRNAs for the second codon of the pair facilitates peptidyl-tRNA shifting t ..."
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The rare codons AGG and AGA comprise 2 % and 4%, respectively, of the arginine codons of Escherichia coli K-12, and their cognate tRNAs are sparse. At tandem occurrences of either rare codon, the paucity of cognate aminoacyl tRNAs for the second codon of the pair facilitates peptidyl-tRNA shifting to the �1 frame. However, AGG_AGG and AGA_AGA are not underrepresented and occur 4 and 42 times, respectively, in E. coli genes. Searches for corresponding occurrences in other bacteria provide no strong support for the functional utilization of frameshifting at these sequences. All sequences tested in their native context showed 1.5 to 11% frameshifting when expressed from multicopy plasmids. A cassette with one of these sequences singly integrated into the chromosome in stringent cells gave 0.9 % frameshifting in contrast to two- to four-times-higher values obtained from multicopy plasmids in stringent cells and eight-times-higher values in relaxed cells. Thus, �1 frameshifting efficiency at AGG_AGG and AGA_AGA is influenced by the mRNA expression level. These tandem rare codons do not occur in highly expressed mRNAs. The expression of a minority of genes in probably all organisms utilizes a change in the translational reading frame at a specific site(s). The ribosomal frameshifting involved occurs at shift-prone sequences, and the proportion of ribosomes that
Stochastic activation of the response regulator PhoB by noncognate histidine kinases. J Integr Bioinform 2: 11. Supporting information Additional supporting information may be found in the online version of this article. Please note: Blackwell Publishing
, 2005
"... Two-component systems (TCS) are the most prevalent gene regulatory mechanism in bacteria. A typical TCS is comprised of a histidine kinase (HK) and a partner response regulator (RR). Specific environment signals lead to autophosphorylation of different HKs, which in turn act as phosphoryl donors for ..."
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Two-component systems (TCS) are the most prevalent gene regulatory mechanism in bacteria. A typical TCS is comprised of a histidine kinase (HK) and a partner response regulator (RR). Specific environment signals lead to autophosphorylation of different HKs, which in turn act as phosphoryl donors for autophosphorylation of their partner RRs. Nonpartner HKs and RRs also interact, giving rise to cross regulation among TCSs in response to diverse signals. PhoR (HK) and PhoB (RR) constitute the TCS for detection of environmental (extracellular) inorganic phosphate (Pi). The PhoR/PhoB TCS controls the expression of a large number of genes for acquisition of alternative phosphorus sources, including phoA, which encodes the non-specific phosphohydrolase bacterial alkaline phosphatase (Bap). Cross activation of PhoB by the nonpartner HK CreC is now a classic example of cross regulation among TCSs. A systematic search for other cross talking HKs revealed five additional HKs that activate (phosphorylate) PhoB (J. M. B. and B. L. W., unpublished data).
