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Fusarium oxysporum as a multihost model for the genetic dissection of fungal virulence in plants and mammals. Infect. Immun
, 2004
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This article cites 35 articles, 16 of which can be accessed free at:
Factors influencing glycosylation of Trichoderma reesei cellulases. Part II: N-glycosylation of Cel7A from different T. reesei strains. Glycobiology, this volume
, 2004
"... The glycosylation of Cel7A (CBH I) from Trichoderma reesei varies considerably when the fungus is grown under different conditions. As shown by ESI-MS and PAG-IEF analyses of both intact protein and the isolated catalytic core module, the microheterogeneity originates mainly from the variable ratio ..."
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The glycosylation of Cel7A (CBH I) from Trichoderma reesei varies considerably when the fungus is grown under different conditions. As shown by ESI-MS and PAG-IEF analyses of both intact protein and the isolated catalytic core module, the microheterogeneity originates mainly from the variable ratio of single N-acetylglucosamine over high-mannose structures on the three N-glycosylation sites and from the presence or absence of phosphate residues. Fully N- and O-glycosylated Cel7A can only be isolated from minimal medium and prob-ably reflects the initial complexity of the protein on leaving the glycosynthetic pathway. Extracellular activities are responsi-ble for postsecretorial modifications in other cultivation con-ditions: a-(1!2)-mannosidase, a-(1!3)-glucosidase and an Endo H type activity participate in N-deglycosylation (core), whereas a phosphatase and a mannosidase are probably responsible for hydrolysis of O-glycans (linker). The effects are most prominent in corn steep liquor–enriched media, where the pH is closer to the pH optimum (5–6) of these extracellular hydrolases. In minimal medium, the low pH and the presence of proteases could explain for the absence of such activities. On the other hand, phosphodiester linkages in the catalytic module are only observed under specific conditions. The extracellular trigger is still unknown, but manno-phosphorylation may be regulated intracellularly by a-(1!2)-mannosidases and phosphomannosyl transferases competing for the same intermediate in the glycosynthetic pathway.
2005. The RIM101/pacC homologue from the basidiomycete Ustilago maydis is functional in multiple pH-sensitive phenomena. Eukaryot. Cell 4:999–1008
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These include: This article cites 42 articles, 23 of which can be accessed free at:
PAC1, a pH-Regulatory Gene from Fusarium verticillioides†
, 2003
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This article cites 37 articles, 16 of which can be accessed free
Biosynthesis and uptake of siderophores is controlled by the PacC-mediated ambient-pH regulatory system
- in Aspergillus nidulans,” Eukaryotic Cell
, 2004
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Growth and Mycotoxin Production by Chaetomium globosum Is Favored in a Neutral pH
, 2008
"... Abstract: Chaetomium globosum is frequently isolated in water-damaged buildings and produces two mycotoxins called chaetoglobosins A and C when cultured on building material. In this study, the influence of ambient pH on the growth of C. globosum was examined on an artificial medium. This fungus was ..."
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Abstract: Chaetomium globosum is frequently isolated in water-damaged buildings and produces two mycotoxins called chaetoglobosins A and C when cultured on building material. In this study, the influence of ambient pH on the growth of C. globosum was examined on an artificial medium. This fungus was capable of growth on potato dextrose agar ranging in pH from 4.3 to 9.4 with optimal growth and chaetoglobosin C production occurring at a neutral pH. In addition, our results show that sporulation is favored in an acidic environment.
Human calpain 7/PalBH associates with a subset of ESCRT-III-related proteins in its N-terminal region and partly localizes to endocytic membrane compartments
- J Biochem
"... Calpain 7 (also known as PalBH) is a mammalian homologue of the Aspergillus, atypical calpain PalB. Knowledge of the biochemical properties of calpain 7 is limited and its function is not yet known. In this study, we investigated the interactions of calpain 7 with all 11 ESCRT-III-related proteins, ..."
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Calpain 7 (also known as PalBH) is a mammalian homologue of the Aspergillus, atypical calpain PalB. Knowledge of the biochemical properties of calpain 7 is limited and its function is not yet known. In this study, we investigated the interactions of calpain 7 with all 11 ESCRT-III-related proteins, named charged multivesicular body proteins (CHMPs), and the subcellular localization of calpain 7. Pulldown assays using stable HEK293T transfectants of Strep-tagged calpain 7 revealed interactions of calpain 7 with a subset of FLAG-tagged CHMPs, among which CHMP1B was selected for further analyses. The N-terminal region containing a tandem repeat of MIT domains of calpain 7 was found to be necessary and sufficient for interaction with CHMP1B. Direct interaction was confirmed by a pulldown assay using recombinant proteins. Fluorescence microscopic analysis using HeLa cells revealed that overexpression of GFP-fused CHMPs or a dominant-negative construct of SKD1/Vps4B caused accumulation of epitope-tagged calpain 7 in a punctate pattern in the perinuclear area. Subcellular fractionation revealed that the most of endogenous calpain 7 is present in the cytosol but a small portion is present
Regulation of Cellulase and Hemicellulase Gene Expression
- in Fungi. Curr Genomics. 2013; 14: 230–249. doi: 10.2174/1389202911314040002 PMID: 24294104
"... Abstract: Research on regulation of cellulases and hemicellulases gene expression may be very useful for increasing the production of these enzymes in their native producers. Mechanisms of gene regulation of cellulase and hemicellulase ex-pression in filamentous fungi have been studied, mainly in As ..."
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Abstract: Research on regulation of cellulases and hemicellulases gene expression may be very useful for increasing the production of these enzymes in their native producers. Mechanisms of gene regulation of cellulase and hemicellulase ex-pression in filamentous fungi have been studied, mainly in Aspergillus and Trichoderma. The production of these ex-tracellular enzymes is an energy-consuming process, so the enzymes are produced only under conditions in which the fungus needs to use plant polymers as an energy and carbon source. Moreover, production of many of these enzymes is coordinately regulated, and induced in the presence of the substrate polymers. In addition to induction by mono- and oligo-saccharides, genes encoding hydrolytic enzymes involved in plant cell wall deconstruction in filamentous fungi can be repressed during growth in the presence of easily metabolizable carbon sources, such as glucose. Carbon catabolite re-pression is an important mechanism to repress the production of plant cell wall degrading enzymes during growth on pre-ferred carbon sources. This manuscript reviews the recent advancements in elucidation of molecular mechanisms respon-sible for regulation of expression of cellulase and hemicellulase genes in fungi.
A genome-wide screen for Neurospora crassa transcription factors regulating glycogen metabolism
"... Transcription factors play a key role in transcription reg-ulation as they recognize and directly bind to defined sites in promoter regions of target genes, and thus modulate differential expression. The overall process is extremely dynamic, as they have to move through the nucleus and transiently b ..."
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Transcription factors play a key role in transcription reg-ulation as they recognize and directly bind to defined sites in promoter regions of target genes, and thus modulate differential expression. The overall process is extremely dynamic, as they have to move through the nucleus and transiently bind to chromatin in order to regulate gene transcription. To identify transcription factors that affect glycogen accumulation in Neurospora crassa, we per-formed a systematic screen of a deletion strains set gen-erated by the Neurospora Knockout Project and available at the Fungal Genetics Stock Center. In a wild-type strain of N. crassa, glycogen content reaches a maximal level at the end of the exponential growth phase, but upon heat stress the glycogen content rapidly drops. The gene en-coding glycogen synthase (gsn) is transcriptionally down-regulated when the mycelium is exposed to the same stress condition. We identified 17 deleted strains having glycogen accumulation profiles different from that of the wild-type strain under both normal growth and heat stress conditions. Most of the transcription factors identified were annotated as hypothetical protein, however some of them, such as the PacC, XlnR, and NIT2 proteins, were biochemically well-characterized either in N. crassa or in other fungi. The identification of some of the transcription factors was coincident with the presence of DNA-binding motifs specific for the transcription factors in the gsn 5-flanking region, and some of these DNA-binding motifs were demonstrated to be functional by Electrophoretic Mo-bility Shift Assay (EMSA) experiments. Strains knocked-out in these transcription factors presented impairment in the regulation of gsn expression, suggesting that the transcrip-tion factors regulate glycogen accumulation by directly reg-ulating gsn gene expression. Five selected mutant strains showed defects in cell cycle progression, and two tran-scription factors were light-regulated. The results indicate that there are connections linking different cellular pro-cesses, such as metabolism control, biological clock, and cell cycle progression. Molecular & Cellular Proteomics
Evidence for Novel pH-Dependent Regulation of Candida albicans Rim101, a Direct Transcriptional Repressor of the Cell Wall
, 2006
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