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Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

by L M Guzman, D Belin, M J Carson, J Beckwith - J , 1995
"... PBAD promoter. arabinoseexpression by vectors containing the Tight regulation, modulation, and high-level ..."
Abstract - Cited by 866 (14 self) - Add to MetaCart
PBAD promoter. arabinoseexpression by vectors containing the Tight regulation, modulation, and high-level

PTP ACTIVITY IS TIGHTLY REGULATED 9

by Università Degli, Studi Di Sassari, Dottorato Di, Ricerca In Biochimica, Biologia E Biotecnologie Molecolari, Coordinatore Prof, Bruno Masala, Prof Francesco, Galimi Dott. Ssa, Elisa Deriu, Anno Accademico
"... ABSTRACT 4 ..."
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ABSTRACT 4

Tightly Regulated Gene Expression System in Salmonella enterica

by Serovar Typhimurium, Sidney Altman , 2002
"... A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P BAD control element into the bacterial chromo ..."
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A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-P BAD control element into the bacterial

Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements

by Rolf Lutz, Hermann Bujard - in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res , 1997
"... Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia coli of several gene activities independently and quantitatively. The novel promoter P LtetO-1 allows ..."
Abstract - Cited by 272 (0 self) - Add to MetaCart
Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia coli of several gene activities independently and quantitatively. The novel promoter P LtetO-1 allows

Tight regulation of unstructured proteins: From transcript synthesis to protein degradation

by Jörg Gsponer, Matthias E. Futschik, Sarah A. Teichmann, M. Madan Babu - Science
"... Altered abundance of several intrinsically unstructured proteins (IUPs) has been associated with perturbed cellular signalling that may lead to pathological conditions such as cancer. Therefore, it is important to understand how cells precisely regulate availability of IUPs. We observe that regulati ..."
Abstract - Cited by 54 (1 self) - Add to MetaCart
Altered abundance of several intrinsically unstructured proteins (IUPs) has been associated with perturbed cellular signalling that may lead to pathological conditions such as cancer. Therefore, it is important to understand how cells precisely regulate availability of IUPs. We observe

A Tightly Regulated Triple Output Asymmetrical Half Bridge Flyback Converter

by Byeong-cheol Hyeon, Bo-hyung Cho
"... In this paper, a tightly regulated triple output asymmetrical half bridge flyback (ASHF) converter is proposed. In order to regulate all output voltages, pulse frequency modulation (PFM), pulse width modulation (PWM) and phase delay (PD) are used simultaneously. In comparison with the conventional P ..."
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In this paper, a tightly regulated triple output asymmetrical half bridge flyback (ASHF) converter is proposed. In order to regulate all output voltages, pulse frequency modulation (PFM), pulse width modulation (PWM) and phase delay (PD) are used simultaneously. In comparison with the conventional

Identifying Tightly Regulated and Variably Expressed Networks by Differential Rank Conservation (DIRAC)

by James A. Eddy, Leroy Hood, Nathan D. Price, Donald Geman
"... A powerful way to separate signal from noise in biology is to convert the molecular data from individual genes or proteins into an analysis of comparative biological network behaviors. One of the limitations of previous network analyses is that they do not take into account the combinatorial nature ..."
Abstract - Cited by 8 (1 self) - Add to MetaCart
or among phenotypes for a selected network. We examined disease phenotypes including cancer subtypes and neurological disorders and identified networks that are tightly regulated, as defined by high conservation of transcript ordering. Interestingly, we observed a strong trend to looser network regulation

Inducible, tightly regulated and growth condition-independent transcription factor in

by Saccharomyces Cerevisiae, Diana S. M. Ottoz, Fabian Rudolf, Joerg Stelling , 2014
"... The precise control of gene expression is essential in basic biological research as well as in biotechnolog-ical applications. Most regulated systems available in yeast enable only the overexpression of the tar-get gene, excluding the possibility of intermediate or weak expression. Moreover, these s ..."
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, called LexA-ER-AD, is tightly regulated by the hormone -estradiol. The selection of the AD proved to be crucial to avoid toxic effects and to define the range of activity that can be precisely tuned with -estradiol. As our sys-tem is based on a heterologous DNA-binding domain, induction in different

Identification of tightly regulated groups of genes during Drosophila melanogaster embryogenesis

by Sean D Hooper, Lars J Jensen, Christopher E Mason, Murad Ghanim, Kevin P White, Eileen Em Furlong, Peer Bork - Mol. Syst. Biol , 2007
"... Time-series analysis of whole-genome expression data during Drosophila melanogaster develop-ment indicates that up to 86 % of its genes change their relative transcript level during embryogenesis. By applying conservative filtering criteria and requiring ‘sharp ’ transcript changes, we identified 15 ..."
Abstract - Cited by 16 (0 self) - Add to MetaCart
1534 maternal genes, 792 transient zygotic genes, and 1053 genes whose transcript levels increase during embryogenesis. Each of these three categories is dominated by groups of genes where all transcript levels increase and/or decrease at similar times, suggesting a common mode of regulation

tightly regulated, so-called Secondary Side Post Regulators

by unknown authors
"... In applications where several output voltages must be ..."
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In applications where several output voltages must be
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