Results 1 - 10 of 1,562

Molecular classification of cancer: class discovery and class prediction by gene expression monitoring

by T. R. Golub, D. K. Slonim, P. Tamayo, C. Huard, M. Gaasenbeek, J. P. Mesirov, H. Coller, M. L. Loh, J. R. Downing, M. A. Caligiuri, C. D. Bloomfield - Science , 1999
"... Although cancer classification has improved over the past 30 years, there has been no general approach for identifying new cancer classes (class discovery) or for assigning tumors to known classes (class prediction). Here, a generic approach to cancer classification based on gene expression monitori ..."
Abstract - Cited by 1779 (19 self) - Add to MetaCart
derived class predictor was able to determine the class of new leukemia cases. The results demonstrate the feasibility of cancer classification based solely on gene expression monitoring and suggest a general strategy for discovering and predicting cancer classes for other types of cancer, independent

The transcriptional program of sporulation in budding yeast

by S. Chu, J. DeRisi, M. Eisen, J. Mulholland, D. Botstein, P. O. Brown, I. Herskowitz - SCIENCE , 1998
"... Diploid cells of budding yeast produce haploid cells through the develop-mental program of sporulation, which consists of meiosis and spore morphogenesis. DNA microarrays containing nearly every yeast gene were used to assay changes in gene expression during sporulation. At least seven distinct temp ..."
Abstract - Cited by 497 (8 self) - Add to MetaCart
temporal patterns of induction were observed. The transcription factor Ndt80 appeared to be important for induction of a large group of genes at the end of meiotic prophase. Consensus sequences known or proposed to be responsible for temporal regulation could be identified solely from analysis of sequences

in 40 ml of AGK 200 [10 mM Hepes (pH

by unknown authors
"... PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY9 ..."
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PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY

in 40 ml of AGK 200 [10 mM Hepes (pH

by unknown authors
"... PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY9 ..."
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PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY

Biological identifications through DNA barcodes

by Paul D. N. Hebert, Alina Cywinska, Shelley L. Ball, Jeremy R. Dewaard - Proc. Biol. Sci , 2003
"... Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon ‘barcodes’. In this study, we establish that ..."
Abstract - Cited by 335 (3 self) - Add to MetaCart
Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon ‘barcodes’. In this study, we establish

A comparison of linkage disequilibrium measures for "ne-scale mapping',

by B Devlin* , Neil Risch - Genomics, , 1995
"... studies is difficult because recombinations are rarely Linkage mapping generally localizes disease genes observed even within the large pedigrees that would to 1-to 2-cM regions of chromosomes. In theory, fur-be required for finer mapping of these loci (Boehnke, ther refinement of location can be a ..."
Abstract - Cited by 219 (9 self) - Add to MetaCart
studies is difficult because recombinations are rarely Linkage mapping generally localizes disease genes observed even within the large pedigrees that would to 1-to 2-cM regions of chromosomes. In theory, fur-be required for finer mapping of these loci (Boehnke, ther refinement of location can

Predicting Protein-Protein Interactions From Primary Structure

by Joel R. Bock, David A. Gough , 2001
"... Motivation: An ambitious goal of proteomics is to elucidate the structure, interactions and functions of all proteins within cells and organisms. The expectation is that this will provide a fuller appreciation of cellular processes and networks at the protein level, ultimately leading to a better un ..."
Abstract - Cited by 191 (4 self) - Add to MetaCart
system was trained to recognize and predict interactions based solely on primary structure and associated physicochemical properties. Results: Inductive accuracy of the trained system, defined here as the percentage of correct protein interaction predictions for previously unseen test sets, averaged 80

Proposal for a new hierarchic classification system, Actinobacteria classis

by Erko Stackebrandt, Fred A. Rainey, Naomi, L. Ward-rainey , 1997
"... A new hierarchic classification structure for the taxa between the taxonomic levels of genus and class is proposed for the actinomycete line of descent as defined by analysis of small subunit (16s) rRNA and genes coding for this molecule (rDNA). While the traditional circumscription of a genus of th ..."
Abstract - Cited by 141 (3 self) - Add to MetaCart
A new hierarchic classification structure for the taxa between the taxonomic levels of genus and class is proposed for the actinomycete line of descent as defined by analysis of small subunit (16s) rRNA and genes coding for this molecule (rDNA). While the traditional circumscription of a genus

Stochasticity in transcriptional regulation: origins, consequences, and mathematical representations

by Thomas B. Kepler, Timothy C. Elston - Biophys. J , 2001
"... ABSTRACT Transcriptional regulation is an inherently noisy process. The origins of this stochastic behavior can be traced to the random transitions among the discrete chemical states of operators that control the transcription rate and to finite number fluctuations in the biochemical reactions for t ..."
Abstract - Cited by 130 (1 self) - Add to MetaCart
impact on the behavior of gene-regulatory networks. We introduce a natural generalization of deterministic bifurcations for classification of stochastic systems and show that simple noisy genetic switches have rich bifurcation structures; among them, bifurcations driven solely by changing the rate

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.

by Willem P C Stemmer , Andreas Crameri , Kim D Ha , Thomas M Brennan , Herbert L Heyneker - Gene , 1995
"... SUMMARY Here, we describe assembly PCR as a method for the synthesis of long DNA sequences from large numbers of oligodeoxyribonucleotides (oligos). The method, which is derived from DNA shuffling [Stemmer, Nature 370 (1994a) 389-391], does not rely on DNA ligase but instead relies on DNA polymer ..."
Abstract - Cited by 115 (0 self) - Add to MetaCart
vector containing the tetracycline-resistance gene (Tc R) as the sole selectable marker. Without relying on ampicillin (Ap) selection, 76% of the Tc R colonies were Ap R, making this approach a general method for the rapid and cost-effective synthesis of any gene. We tested the range of assembly PCR
Results 1 - 10 of 1,562