We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation

” between 37 Internet sites. We analyze the routing behavior for pathological conditions, routing stability, and routing symmetry. For pathologies, we characterize the prevalence of routing loops, erroneous routing, infrastructure failures, and temporary outages. We find that the likelihood of encountering

DNA cytosine methylation is a central epigenetic modification that has essential roles in cellular processes including genome regulation, development and disease. Here we present the first genome-wide, single-base-resolution maps of methylated cytosines in a mammalian genome, from both human

We propose novel cooperative transmission protocols for delay-limited coherent fading channels consisting of (half-duplex and single-antenna) partners and one cell site. In our work, we differentiate between the relay, cooperative broadcast (down-link), and cooperative multiple-access (CMA) (up

The Web has become an excellent source for gathering consumer opinions. There are now numerous Web sites containing such opinions, e.g., customer reviews of products, forums, discussion groups, and blogs. This paper focuses on online customer reviews of products. It makes two contributions. First

to explore the solution space and makes the split and merge process reversible at a middle level vision formulation. Thus it achieves globally optimal solution independent of initial segmentations. Secondly, instead of computing a single maximum a posteriori solution, it proposes a mathematical principle

of different rates at a single site. Thus with 3 rates, the effort involved is no greater than 3 times that for a single rate. This "hidden Markov model" method allows for rates to differ between sites, and for correlations between the rates of neighboring sites. By summing over all possibilities

that this is the most common mechanism of duplicate-gene preservation. An alternative hypothesis is that gene duplicates are frequently preserved by subfunctionalization, whereby both members of a pair experience degenerative mutations that reduce their joint levels and patterns of activity to that of the single

Component failure in large-scale IT installations is becoming an ever larger problem as the number of components in a single cluster approaches a million. In this paper, we present and analyze field-gathered disk replacement data from a number of large production systems, including high

InterPro, an integrated documentation resource of protein families, domains and functional sites, was created in 1999 as a means of amalgamating the major protein signature databases into one comprehensive resource. PROSITE, Pfam, PRINTS, ProDom, SMART and TIGRFAMs have been manually integrated