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Tips and Tools Restriction Enzyme Digestion Exercise – An In-class Activity
"... Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and ..."
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Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and
Use of a restriction enzyme-digested PCR product as substrate for helicase assays
, 2004
"... DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is desc ..."
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is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated.
RESTRICTION ENZYME DIGESTION ANALYSIS OF PCR-AMPLIFIED DNA OF BLASTOCYSTIS HOMINIS ISOLATES
, 2007
"... Abstract. Genomic DNA of Blastocystis isolates released into 0.1 % Triton X-100 was suitable for amplification and yielded similar results as the genomic DNA extracted with standard kit. The specific B. hominis primers (BH1: GCT TAT CTG GTT GAT CCT GCC AGT and BH2: TGA TCC TTC CGC AGG TTC ACC TAC A) ..."
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) successfully produced the PCR product of about 1,770 bp with all the 7 Blastocystis isolates tested. The restriction fragment length polymorphism (RFLP) patterns yielded by 13 out of 25 restriction endonucleases showed that the 7 isolates could be grouped into 4 subgroups: subgroup-1 consisted of isolate C
Use of PCR amplification and restriction enzyme digestion of mitochondrial D-loop for identification of mustelids in Ireland
"... stoat). A DNA test has been developed to unambiguously identify scat or hair samples by amplification and restriction enzyme analysis of the mitochondrial D-loop region. Sequencing revealed one haplotype for pine marten (Hap p), two for otter (Lut 1 and 2), three novel haplotypes for mink, and one f ..."
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stoat). A DNA test has been developed to unambiguously identify scat or hair samples by amplification and restriction enzyme analysis of the mitochondrial D-loop region. Sequencing revealed one haplotype for pine marten (Hap p), two for otter (Lut 1 and 2), three novel haplotypes for mink, and one
IDENTIFICATION OF THREE ARMYWORM SPECIES (LEPIDOPTERA: NOCTUIDAE) USING DNA BARCODES AND RESTRICTION ENZYME DIGESTION
, 2010
"... Abstract SIX SPECIES of sugarcane armyworms (Mythimna spp.) are known to occur in Mauritius, namely M. pseudoloreyi, M. loreyi, M. insulicola, M. phaea, M. tincta and M. pyrausta. Due to the close resemblance of the adult moths, morphological distinction among the different species is difficult ..."
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different body parts including abdomen, leg and wing. A ~700 bp PCR fragment amplified from the three species, using primers HCO2198/LCO1490, was digested using restriction enzymes. A combination of four enzymes Rsa1, TaqI, PvuII and SacI successfully allowed distinction of the three species of armyworms
analysis of factor V Leiden and prothrombin 20210GA. A comparison of the Nanogen Eelectronic Microarray with restriction enzyme digestion and
"... We are grateful to Biosense for supplying the instrument ..."
Evaluation of Transcriptional Efficiency of Hepatitis B Virus Covalently Closed Circular DNA by Reverse Transcription-PCR Combined with the Restriction Enzyme Digestion Method†
, 2004
"... Updated information and services can be found at: ..."
Characterization of Biomphalaria orbignyi, Biomphalaria peregrina and Biomphalaria oligoza by Polymerase Chain Reaction and Restriction Enzyme Digestion of the Internal Transcribed Spacer Region of the RNA Ribosomal Gene
"... The correct identification of Biomphalaria oligoza, B. orbignyi and B. peregrina species is difficult due to the morphological similarities among them. B. peregrina is widely distributed in South America and is considered a potential intermediate host of Schistosoma mansoni. We have reported the use ..."
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. The restriction patterns obtained with MvaI enzyme presented the best profile to identify the three species. The profiles obtained with all enzymes were used to estimate genetic similarities among B.
Detection of Restriction Enzyme–Digested Target DNA by PCR Amplification Using a Stem-Loop Primer: Application to the Detection of Hypomethylated Fetal DNA in Maternal Plasma
"... maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis and monitoring. Among the fetal markers that have been described, methylation markers are sex and polymorphism independent. Methyl-ation-sensitive restriction endonucleases are commonly used to digest hypomethylated D ..."
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maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis and monitoring. Among the fetal markers that have been described, methylation markers are sex and polymorphism independent. Methyl-ation-sensitive restriction endonucleases are commonly used to digest hypomethylated
Type Package Title Analysis of high-throughput sequencing data processed by restriction enzyme digestion Version 1.6.0 Date 2012-05-23
, 2013
"... Description The package includes functions to build restriction enzyme cut site (RECS) map, dis-tribute mapped sequences on the map with five different approaches, find en-riched/depleted RECSs for a sample, and identify differentially enriched/depleted RECSs be-tween samples. License GPL (>=2) R ..."
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Description The package includes functions to build restriction enzyme cut site (RECS) map, dis-tribute mapped sequences on the map with five different approaches, find en-riched/depleted RECSs for a sample, and identify differentially enriched/depleted RECSs be-tween samples. License GPL (>=2
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