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ORIGINAL PAPER In-Depth Characterisation of Retinal Pigment Epithelium (RPE) Cells Derived from Human Induced Pluripotent Stem Cells (hiPSC)
"... The Author(s) 2014. This article is published with open access at Springerlink.com Abstract Induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium (RPE) has widely been appre-ciated as a promising tool to model human ocular disease emanating from primary RPE pathology. Here, we des ..."
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describe the successful reprogramming of adult human dermal fibroblasts to iPSCs and their differentiation to pure expandable RPE cells with structural and functional fea-tures characteristic for native RPE. Fibroblast cultures were established from skin biopsy material and subsequently reprogrammed
RELATIVE PERFORMANCE EVALUATION WITH SYSTEMATIC
"... Most observed managerial compensation packages do not filter out systematic economy-wide fluctuations (e.g., market indices, oil prices, macroeconomic variables). However, given that such shocks do not appear informative on individual actions, the limited use of relative performance evaluation (RPE) ..."
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wealth and thus greater risk tolerance. Under constant relative risk-aversion, a contract with pure RPE is suboptimal even if the manager can privately trade the market. The framework suggests that apparent rejections of RPE may be driven by rational asset pricing effects, and offers an empirical
LUMINESCENCE AND TRAPPING CENTRES IN PURE AND Cd DOPED SILVER CHLORIDE CRYSTALS (*)
"... Résumé. — Nous avons étudié par luminescence, thermoluminescence et RPE la nature du centre de la luminescence dans les monocristaux de chlorure d'argent purs et dopés avec Cd2+. Les résultats montrent, que le centre Ag2+ (trou autopiégé) est un centre efficace de luminescence seulement au-dess ..."
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Résumé. — Nous avons étudié par luminescence, thermoluminescence et RPE la nature du centre de la luminescence dans les monocristaux de chlorure d'argent purs et dopés avec Cd2+. Les résultats montrent, que le centre Ag2+ (trou autopiégé) est un centre efficace de luminescence seulement au
Evaluation of the rhodopsin knockout mouse as a model of pure cone function. Invest Ophthalmol Vis Sci 42
, 2001
"... PURPOSE. To determine a time window in the rhodopsin knockout (Rho Ϫ/Ϫ ) mouse during which retinal function is already sufficiently developed but cone degeneration is not yet substantial, thus representing an all-cone retina. METHODS. Electroretinograms (ERGs) were obtained from 14 homozygous Rho ..."
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epithelium (RPE) and choroid did not show any abnormalities. CONCLUSIONS. From PW4 to 6, Rho Ϫ/Ϫ mice appear to have normal cone and no rod function. Despite the missing rod outer segment (OS), the structure of retina, RPE, and choroid remained unchanged. Therefore, the Rho Ϫ/Ϫ mice can serve during this age
unknown title
"... The retinal pigment epithelium (RPE) is a single layer of pigmented epithelial cells that supports the function and development of photoreceptors [1]. RPE dysfunction leads to many retinal degenerative diseases, including age-related macular degeneration (AMD) [2] and retinitis pigmentosa (RP) [3,4] ..."
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-tation in studying RPE signaling is the difficulty obtaining pure and intact RPE tissue from small developing embryos, which has precluded accurate expression profiling of the RPE during development. We previously addressed this issue by developing an approach for estimating the RPE gene expres-sion profile
Nutritional Manipulation of Primate Retinas, II: Effects of Age, n–3 Fatty Acids, Lutein, and Zeaxanthin on Retinal Pigment Epithelium
"... PURPOSE. To study the effects of age and of n–3 fatty acids, lutein, and zeaxanthin on the retinal pigment epithelium (RPE). METHODS. Rhesus monkeys (age range, 7–17 years; n � 18) were fed xanthophyll-free semipurified diets from birth. The diets had either low or adequate amounts of n–3 fatty acid ..."
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acids. Six monkeys remained xanthophyll-free until death. Six received supplements of pure lutein and six of pure zeaxanthin for 6 to 24 months. The central retina was serially sectioned, and the number of RPE cells were counted in an 8-�m strip along the vertical meridian. Cell counts were compared
286 © 2015 Journal of ophthalmic and Vision research | published by Wolters KluWer- medKnoW Behavior of a Spontaneously Arising Human Retinal Pigment Epithelial Cell Line Cultivated on Thin Alginate Film
"... Purpose: A cell line spontaneously derived from human retinal pigment epithelium (hRPE) was cultured on alginate film gelatinized with different concentrations of neurobasal cell culture medium (NCCM) to assess its growth and morphological behavior on this naturally occurring polysaccharide. Methods ..."
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‑coated microplates. After 5 days, hRPE colonies were harvested and re‑plated on polystyrene substrates. Morphology and growth of hRPE cultures were determined during the next 2 weeks. Results: The first few passages of the cultures were purely hRPE cells that revealed typical morphological features of the pigmented
Retinal Pigment Epithelium Culture; a Potential Source of Retinal Stem Cells
"... Purpose: To establish human retinal pigment epithelial (RPE) cell culture as a source for cell replacement therapy in ocular diseases. Methods: Human cadaver globes were used to isolate RPE cells. Each globe was cut into several pieces of a few millimeters in size. After removing the sclera and chor ..."
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Purpose: To establish human retinal pigment epithelial (RPE) cell culture as a source for cell replacement therapy in ocular diseases. Methods: Human cadaver globes were used to isolate RPE cells. Each globe was cut into several pieces of a few millimeters in size. After removing the sclera
Q and Zheng H: Migration of retinal pigment epithelium cells is regulated by protein kinase Calpha in vitro. Invest Ophthalmol Vis Sci 54
, 2013
"... PURPOSE. Retinal pigment epithelium (RPE) cell migration and proliferation are considered key elements in proliferative vitreoretinopathy (PVR). Downregulation of protein kinase Ca (PKCa) can inhibit RPE cell proliferation. Here, we sought to analyze whether PKCa affects the migration of RPE cells. ..."
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was confirmed by quantitative RT-PCR (qRT-PCR). Cell migration ability was analyzed by wound healing assay and transwell chamber assay. Expression of zonula occludens (ZO)-1 and occludin was determined by immunofluorescence. RESULTS. Pure populations of hRPE cell cultures were observed using light
Effects of lipid peroxidation-related protein modifications
- on RPE lysosomal functions and POS
, 2007
"... PURPOSE. Lipofuscin accumulation in the RPE is a common downstream pathogenic pathway in various monogenic and complex retinal diseases including age-related macular degeneration (AMD). Lipid peroxidation-induced modification of proteins is thought to play a role in lipofuscinogenesis and may contr ..."
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Cited by 4 (0 self)
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to proteolytic attack and act as protease inhibitors. METHODS. Isolated photoreceptor outer segments (POS) were radioactively labeled and in vitro modified with MDA and HNE. Pure lysosomal fractions isolated from human RPE were tested for their proteolytic activities toward modified and unmodified POS proteins
Results 1 - 10
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13
