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An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database

by Jimmy K. Eng, Ashley L. Mccormack, John R. Yates - J. Am. Soc. Mass Spectrom , 1994
"... A method to correlate the uninterpreted tandem mass spectra of peptides produced under low energy (lo-50 eV) collision conditions with amino acid sequences in the Genpept database has been developed. In this method the protein database is searched to identify linear amino acid sequences within a mas ..."
Abstract - Cited by 944 (19 self) - Add to MetaCart
mass tolerance of * 1 u of the precursor ion molecular weight. A cross-correlation function is then used to provide a measurement of similarity between the mass-to-charge ratios for the fragment ions predicted from amino acid sequences obtained from the database and the fragment ions observed

for precursors

by Wayne Martindale, Martin A. J. Parry, P. John Andralojc, Alfred J. Keys , 1996
"... Discs of French bean leaves were vacuum infiltrated with solutions containing 14C-labelled substances. The infiltrated discs were either transferred immediately to darkness or first illuminated for 2 h and then transferred to darkness. After 6 h in darkness the discs were extracted with buffer conta ..."
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containing CO2, Mg2 + and additional ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco; EC 4.1.1.39). Protein in the extracts was separated from substances of low molecular weight by gel filtration and coagulated by heating to 100°C. Coagulated protein was removed by centrifugation and cations

Communications to the Editor Effect of Molecular Weight of the Reactive Precursors in Melt Reactive Blending

by C. W. Macosko , 2002
"... Introduction. Immiscible polymer blends are com-monly compatibilized by block or graft copolymers, which are either premade or formed “in situ”.1 The reactive blending process is by far more economically viable but also strongly dependent on the relative ..."
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Introduction. Immiscible polymer blends are com-monly compatibilized by block or graft copolymers, which are either premade or formed “in situ”.1 The reactive blending process is by far more economically viable but also strongly dependent on the relative

Molecular Weight Reverse Osmosis

by unknown authors
"... hen altering the recycling 01 waste treatment process within ing system, savvy man1 facturers will benefit from.oming their own “experts ” n recycling technology. Peynolds aning industry, much of the data ion about products and services r.ed by vendors and individuals.endors. This is not to say that ..."
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hen altering the recycling 01 waste treatment process within ing system, savvy man1 facturers will benefit from.oming their own “experts ” n recycling technology. Peynolds aning industry, much of the data ion about products and services r.ed by vendors and individuals.endors. This is not to say

H.: LowMolecular-Weight and Oligomeric Components in Secondary Organic Aerosol from the Ozonolysis of Cycloalkenes and αPinene

by Song Gao, Melita Keywood, Nga L. Ng, Jason Surratt, Varuntida Varutbangkul, Roya Bahreini, Richard C. Flagan, John H. Seinfeld - J. Phys. Chem , 2004
"... The composition of secondary organic aerosol (SOA) from the ozonolysis of C5-C8 cycloalkenes and R-pinene, as well as the effects of hydrocarbon precursor structure and particle-phase acidity on SOA formation, have been investigated by a series of controlled laboratory chamber experiments. A liquid ..."
Abstract - Cited by 16 (5 self) - Add to MetaCart
chromatography-mass spectrometer and an ion trap mass spectrometer are used concurrently to identify and to quantify SOA components with molecular weights up to 1600 Da. Diacids, carbonyl-containing acids, diacid alkyl esters, and hydroxy diacids are the four major classes of low-molecular-weight (MW < 250 Da

Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processings, and complete amino acid sequences

by Louis E. Henderson, Michelle A. Bowers, Raymond C. Sowder Ii, Stefan A. Serabyn, Donald G. Johnson, Julian W. Bess, Larry Arthur, Duncan K. Bryant, Catherine Fenselau , 1992
"... The MN strain of human immunodeficiency virus type 1 was grown in H9 cells, concentrated by centrifugation, and disrupted, and proteins were purified by reversed-phase high-pressure liquid chromatog-raphy. Complete amino acid sequences were determined for the mature Gag proteins, showing natural pro ..."
Abstract - Cited by 83 (12 self) - Add to MetaCart
but differ from the precursors predicted by the published MN proviral DNA sequence by 10 residues. Electrospray ionization mass spectrometry analysis of the purified p24 forms showed that the measured molecular weight of the protein was 200 ± 50 atomic mass units greater than the calculated molecular weight

Adsorption of Low-molecular-weight Sodium Polyacrylate on Hydroxyapatite

by D. N. Misra
"... Adsorption of low-molecular-weight sodium polyacrylate from aqueous solution onto synthetic hydroxyapatite was studied at room temperature so that the mechanism of adhesion of polyacrylate cements to tooth mineral could be elucidated. The adsorption isotherm of sodium polyacrylate was Langmuirian in ..."
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Adsorption of low-molecular-weight sodium polyacrylate from aqueous solution onto synthetic hydroxyapatite was studied at room temperature so that the mechanism of adhesion of polyacrylate cements to tooth mineral could be elucidated. The adsorption isotherm of sodium polyacrylate was Langmuirian

Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83,000 tool wt form expressed on the surface of Plasmodiumfalciparum merozoites

by R. Freeman, Anthony, A. Holder , 1983
"... Immunity against blood stage Plasmodium falciparum malaria can be passively transferred with immune gamma globulin (1), and protective antibodies appear to act against the merozoite form of the parasite (2). For vaccine development, therefore, it is important to identify and characterize the surface ..."
Abstract - Cited by 8 (2 self) - Add to MetaCart
's intraerythrocytic growth cycle (3). The major processing product detected after merozoite differentiation was an 83,000 mol wt fragment, and it was suggested that this fragment of the •95,000 tool wt precursor may be expressed on the merozoite surface. More recently, we have demonstrated a serological cross

LYSOSOMAL HIGH MOLECULAR WEIGHT MULTIENZYME COMPLEX

by Halina Ostrowska, Katarzyna Krukowska, Mirosława Orłowska, Ilona Lengiewicz
"... sulfatase (GALNS, EC 3.1.6.4) and serine carboxypepidase cathepsin A (EC 3.4.16.1) form a functional high molecular weight complex in the lysosomes. The major constituent of this complex is cathepsin A, the so-called “lysosomal protective protein ” (PPCA). By forming a multienzyme complex, it protec ..."
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sulfatase (GALNS, EC 3.1.6.4) and serine carboxypepidase cathepsin A (EC 3.4.16.1) form a functional high molecular weight complex in the lysosomes. The major constituent of this complex is cathepsin A, the so-called “lysosomal protective protein ” (PPCA). By forming a multienzyme complex

Clarifying the Molecular Weight Dependence of the Segmental Dynamics

by Of Polybutadiene , 2009
"... ABSTRACT: A series of 1,4-polybutadienes of varying molecular weight, both monodisperse and having broad or bidisperse molecular weight distributions, were studied using dielectric relaxation. The glass transition temperature,Tg, and theTg-normalized temperature dependence of the segmental relaxatio ..."
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ABSTRACT: A series of 1,4-polybutadienes of varying molecular weight, both monodisperse and having broad or bidisperse molecular weight distributions, were studied using dielectric relaxation. The glass transition temperature,Tg, and theTg-normalized temperature dependence of the segmental
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