Results 1 - 10 of 7,290

Three-Dimensional Structure of the Neisseria meningitidis Secretin PilQ Determined from Negative-Stain Transmission Electron Microscopy

by Tone Tønjum, Jeremy P. Derrick , 2002
"... The PilQ secretin from the pathogenic bacterium Neisseria meningitidis is an integral outer membrane protein complex which plays a crucial role in the biogenesis of type IV pili. We present here the first three-dimensional structure of this type of secretin at 2.5-nm resolution, obtained by single-p ..."
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-particle averaging methods applied to the purified protein complex visualized in a negative stain. In projection, the PilQ complex is circular, with a donut-like appearance. When viewed from the side it has a rounded, conical profile. The complex was demonstrated to have 12-fold rotational symmetry

Three-Dimensional Structure of the Neisseria meningitidis Secretin PilQ Determined from Negative-Stain Transmission Electron Microscopy

by Neisseria Meningitidis Secretin Pilq, Richard F. Collins, Robert C. Ford, Ashraf Kitmitto, O. Olsen, Tone Tønjum, Jeremy P. Derrick, J. Bacteriol, Richard F. Collins, Robert C. Ford, Ashraf Kitmitto, Ranveig O. Olsen, Tone Tønjum, Jeremy P. Derrick , 2002
"... Updated information and services can be found at: ..."
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Updated information and services can be found at:

Negative-Staining Electron Microscopy†

by Mycoplasma Gallisepticum, Daisuke Nakane, Makoto Miyata , 2008
"... Several mycoplasma species feature a membrane protrusion at a cell pole, and unknown mechanisms provide gliding motility in the direction of the pole defined by the protrusion. Mycoplasma gallisepticum, an avian pathogen, is known to form a membrane protrusion composed of bleb and infrableb and to g ..."
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pneumoniae. Next, to elucidate the cytoskeletal structure of M. gallisepticum, we stripped the envelopes by treatment with Triton X-100 under various condi-tions and observed the remaining structure by negative-staining transmission electron microscopy. A unique cytoskeletal structure, about 300 nm long

ISSN 1684–5315 © 2009 Academic Journals

by unknown authors
"... Ultramicroscopic observation of recombinant adeno-associated virus type 2 on the surface of formvar-carbon coated copper grids under different relative humidity and incubation time using negative stain transmission electron microscopy ..."
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Ultramicroscopic observation of recombinant adeno-associated virus type 2 on the surface of formvar-carbon coated copper grids under different relative humidity and incubation time using negative stain transmission electron microscopy

Transmission Electron Microscopy, Scanning Tunneling Microscopy, and Atomic Force Microscopy of the Cell Envelope Layers of the Archaeobacterium

by Methanospirillum Hungatei Gp, G. Southam, M. Firtel, B. L. Blackford, M. H. Jericho, W. Xu, P. J. Mulhern, T. J. Beveridge , 1992
"... Methanospirllum hungatei GP1 possesses paracrystalline cell envelope components including end plugs and a sheath formed from stacked hoops. Both negative-stain transmission electron microscopy (TEM) and scanning tunneling microscopy (STM) distinguished the 2.8-nm repeat on the outer surface of the s ..."
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Methanospirllum hungatei GP1 possesses paracrystalline cell envelope components including end plugs and a sheath formed from stacked hoops. Both negative-stain transmission electron microscopy (TEM) and scanning tunneling microscopy (STM) distinguished the 2.8-nm repeat on the outer surface

Frog Oocytes to Unveil the Structure and Supramolecular Organization of Human Transport Proteins

by Marc J. Bergeron, Rajendra Boggavarapu, Marcel Meury, Zöhre Ucurum, Luc Caron, Paul Isenring, Matthias A. Hediger, Dimitrios Fotiadis , 2011
"... Structural analyses of heterologously expressed mammalian membrane proteins remain a great challenge given that microgram to milligram amounts of correctly folded and highly purified proteins are required. Here, we present a novel method for the expression and affinity purification of recombinant ma ..."
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mammalian and in particular human transport proteins in Xenopus laevis frog oocytes. The method was validated for four human and one murine transporter. Negative stain transmission electron microscopy (TEM) and single particle analysis (SPA) of two of these transporters, i.e., the potassiumchloride

MagicWand: A Single, Designed Peptide That Assembles to Stable, Ordered

by unknown authors , 2008
"... ABSTRACT: We describe a straightforward single-peptide design that self-assembles into extended and thickened nano-to-mesoscale fibers of remarkable stability and order. The basic chassis of the design is the well-understood dimeric R-helical coiled-coil motif. As such, the peptide has a heptad sequ ..."
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unfolding of MW is reversible with a melting temperature>70 °C at 100 µM peptide concentration. Negative-stain transmission electron microscopy (TEM) of MW assemblies reveals stiff, straight, fibrous rods that extended for tens of microns. Moreover, different stains highlight considerable order both

Characterization of Starburst Dendrimers and Vesicle Solutions and Their Interactions by CW- and Pulsed-EPR, TEM, and Dynamic Light Scattering

by M. Francesca Ottaviani, Paolo Matteini, Marina Brustolon, Nicholas J. Turro, Steffen Jockusch, Donald A. Tomalia , 1998
"... Starburst dendrimers (SBDs) of different generations (size) and level of protonations of their surface amino groups and solutions of dimyristoylphosphatidylcholine (DMPC) vesicles were analyzed, both separately and in mixtures of the two components, by negative-staining transmission electron microsc ..."
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Starburst dendrimers (SBDs) of different generations (size) and level of protonations of their surface amino groups and solutions of dimyristoylphosphatidylcholine (DMPC) vesicles were analyzed, both separately and in mixtures of the two components, by negative-staining transmission electron

Detergent-Induced Stabilization and Improved 3D Map of the Human Heteromeric Amino Acid Transporter 4F2hc-LAT2

by Marcel Meury, Meritxell Costa, Daniel Harder, Mirko Stauffer, Jean-marc Jeckelmann, Albert Rosell, Karin Kovar, Dimitrios Fotiadis
"... Human heteromeric amino acid transporters (HATs) are membrane protein complexes that facilitate the transport of specific amino acids across cell membranes. Loss of function or overexpression of these transporters is implicated in several human diseases such as renal aminoacidurias and cancer. HATs ..."
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. Microgram amounts of purified protein made possible the reconstruction of the first 3D map of a human HAT by negative-stain transmission electron microscopy. Here we report the important stabilization of purified human 4F2hc-LAT2 using a combination of two detergents, i.e., n

Phase Behavior and Spontaneous Vesicle Formation in Aqueous Solutions of Anionic Ammonium Dodecyl Sulfate and Cationic Octadecyl Trimethyl Ammonium

by Chloride Surfactants, Kye-hong Kang, Hong-un Kim, Kyung-hee Lim , 2007
"... Phase behavior for the mixed aqueous surfactant systems of cationic octadecyl trimethyl ammonium chloride (OTAC)/anionic ammonium dodecyl sulfate (ADS)/water was examined. Below the total surfactant concentrations of 1.5 m molal, mixed micelles were formed. At the total surfactant concentrations hig ..."
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higher than 1.5 m molal, there appeared a region where mixed micelles and vesicles coexist. As the surfactant concentration increased, the systems looked very turbid and much more vesicles were observed. The vesicles were spontaneously formed in this system and their existence was observed by negative-staining
Results 1 - 10 of 7,290