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Results 11 - 20
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100
Modeling Formamide Denaturation of Probe-Target Hybrids for Improved Microarray Probe Design in Microbial Diagnostics
"... Application of high-density microarrays to the diagnostic analysis of microbial communities is challenged by the optimization of oligonucleotide probe sensitivity and specificity, as it is generally unfeasible to experimentally test thousands of probes. This study investigated the adjustment of hybr ..."
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of hybridization stringency using formamide with the idea that sensitivity and specificity can be optimized during probe design if the hybridization efficiency of oligonucleotides with target and non-target molecules can be predicted as a function of formamide concentration. Sigmoidal denaturation profiles were
Population Analysis in a Denitrifying Sand Filter: Conventional and In Situ Identification of Paracoccus spp. in
, 1996
"... The microbial community of a denitrifying sand filter in a municipal wastewater treatment plant was examined by conventional and molecular techniques to identify the bacteria actively involved in the removal of nitrate. In this system, denitrification is carried out as the last step of water treatme ..."
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Cited by 16 (1 self)
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were designed for this genus and the species cluster Paracoccus denitrificans-Paracoccus versutus and tested for specificity by whole-cell hybridization. Stringency requirements for the probes were adjusted by use of a formamide concentration gradient to achieve complete discrimination of even highly
Reaction Temperature Constraints in DNA Computing
"... Using the thermodynamics of DNA melting, a technique is proposed to choose a reaction temperature for the DNA computation that minimizes the potential for mishybridizations. Adleman[Adleman, 1994] showed the computational potential of the hybridization reaction and other molecular biology protocols. ..."
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as the hybridization stringency. In general, as the reaction temperature of the hybridization is increased up to a critical point, the stringency increases. The temperature at which half the population of perfectly matched
Genomic In Situ Hybridization (GISH) as a Tool to Identify Chromosomes of Parental Species in Sunflower Interspecific Hybrids
"... Interspecific hybridization has been widely used to transfer genes from wild species into cultivated sunflower. Fluorescent genomic in situ hybridization (GISH) has been used to identify alien chromosomes or segments in other crops, but an equivalent technique for sunflower is lacking. The objective ..."
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Interspecific hybridization has been widely used to transfer genes from wild species into cultivated sunflower. Fluorescent genomic in situ hybridization (GISH) has been used to identify alien chromosomes or segments in other crops, but an equivalent technique for sunflower is lacking
Histone gene number and organisation in Xenopus: Xenopus borealis has a homogeneous major duster
, 1983
"... Using a Xenopus laevis H4 cDNA clone as a probe we have determined that the numbers of H4 histone genes in Xenopus laevis and Xenopus borealis are approximately the same. These numbers are dependent on the hybridization stringency and we measure about 90 H4 genes per haploid genome after a 60°C wash ..."
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Using a Xenopus laevis H4 cDNA clone as a probe we have determined that the numbers of H4 histone genes in Xenopus laevis and Xenopus borealis are approximately the same. These numbers are dependent on the hybridization stringency and we measure about 90 H4 genes per haploid genome after a 60°C
UNIT 2.10 Hybridization Analysis of DNA Blots The
"... principle of hybridization analysis is that a single-stranded DNA or RNA molecule of defined sequence (the “probe”) can base-pair to a second DNA or RNA molecule that contains a complementary sequence (the “target”), with the stability of the hybrid depending on the extent of base pairing that occur ..."
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hybridization. After a suitable incubation, the membrane is washed so that any nonspecifically bound probe is removed, leaving only probe that is base-paired to the target DNA. By controlling the stringency of the washing conditions, decisions can be made about whether to target sequences that are 100
Identification of a new genogroup of aquareovirus by RNA–RNA hybridization
- Journal of General Virology
, 1997
"... The relative mobilities of the 11 dsRNA genomic segments of 22 aquareovirus isolates from fish and shellfishobtained fromdifferent geographical areas of the world were compared by PAGE. Using reciprocal RNA–RNA dot blot hybridization, a new sixth genetic group of aquareovirus (genogroup F) was ident ..."
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Cited by 1 (1 self)
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The relative mobilities of the 11 dsRNA genomic segments of 22 aquareovirus isolates from fish and shellfishobtained fromdifferent geographical areas of the world were compared by PAGE. Using reciprocal RNA–RNA dot blot hybridization, a new sixth genetic group of aquareovirus (genogroup F
Copyright C 1992, American Society for Microbiology Characterization of Full-Length and Polymerase Chain
, 1992
"... To determine the VP4 (P type) specificity of porcine rotaviruses, full- and partial-length gene 4 probes were produced from cloned Gottfried and OSU porcine rotavirus genomic segment 4 cDNAs. The gene 4 segments from the prototype Gottfried (VP7 serotype 4) and OSU (VP7 serotype 5) porcine rotavirus ..."
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to 612) of VP8 encoded by genomic segment 4. The hybridization stringency conditions necessary for optimal probe specificity and sensitivity were determined by dot or Northern (RNA) blot hybridizations against a diverse group of human and animal rotaviruses of heterologous group A serotypes and against
Expanding probe repertoire and improving reproducibility in human genomic hybridization
, 2012
"... Diagnostic DNA hybridization relies on probes composed of single copy (sc) genomic sequences. Sc sequences in probe design ensure high specificity and avoid cross-hybridization to other regions of the genome, which could lead to ambiguous results that are difficult to interpret. We examine how the d ..."
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Cited by 2 (0 self)
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the distri-bution and composition of repetitive sequences in the genome affects sc probe performance. A divide and conquer algorithm was implemented to design sc probes. With this approach, sc probes can include divergent repetitive elements, which hybridize to unique genomic targets under higher stringency
The human c-Ha-nzs2 is a processed pseudogene inactivated by numerous base substitutions
, 1984
"... The human c-Ha-ras2 gene, one of two known members of the Harvey ras family, ia reportedly located on the X-chromosome and has lost introns (1, 2). There has heretofore been no information on its precise gene structure and oncogenic potential. He have determined the nucleotide sequence of the c-Ha-r ..."
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genomic DNA library using the v-Ha-ras probe at various hybridization stringency. Among these clones, we report here the nucleotide sequence of the c-Ha-ras2 and demonstrated that it is a processed and inactivated pseudogene accompanied by several direct repeats.
Results 11 - 20
of
100
