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100
A PCR-based method for high stringency screening of DNA libraries. Nucleic Acids Res
, 1993
"... A rapid method for cloning genomlc DNA utilizing a PCR-based screening protocol Is described. A murlne genomic library In lambda phage was subdivided Into 64 wells, each containing 1000 clones, and propagated In bacteria. Amplified phage from each of 8 wells across columns, and each of 8 wells down ..."
Abstract
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Cited by 13 (1 self)
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rows, were pooled. The pooled phage were screened for the presence of murine M-CSF DNA by PCR using specific ollgonucleotide primers. A single well that contained an M-CSF genomlc clone was identified by the synthesis of a PCR product of the correct size that hybridized to an internal M
NANO IDEA Open Access SPR Detection and Discrimination of the Oligonucleotides Related to the Normal and the Hybrid bcr-abl Genes by Two Stringency Control Strategies
"... In this study, we applied two stringency control strategies for surface plasmon resonance (SPR) detection of DNA hybridization and discrimination of completely and partially complementary 24-mer sequences. These sequences are specific to the human normal bcr and the hybrid bcr-abl genes, protein pro ..."
Abstract
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In this study, we applied two stringency control strategies for surface plasmon resonance (SPR) detection of DNA hybridization and discrimination of completely and partially complementary 24-mer sequences. These sequences are specific to the human normal bcr and the hybrid bcr-abl genes, protein
Electrokinetic stringency control in self-assembled monolayer-based biosensors for multiplex urinary tract infection diagnosis
"... Abstract Rapid detection of bacterial pathogens is critical toward judicious management of infectious diseases. Herein, we demonstrate an in situ electrokinetic stringency control approach for a self-assembled monolayer-based electrochemical biosensor toward urinary tract infection diagnosis. The i ..."
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. The in situ electrokinetic stringency control technique generates Joule heating induced temperature rise and electrothermal fluid motion directly on the sensor to improve its performance for detecting bacterial 16S rRNA, a phylogenetic biomarker. The dependence of the hybridization efficiency reveals
Connexin43: a protein from rat heart homologous to a gap junction protein from liver
- J. Cell
, 1987
"... Abstract. Northern blot analysis of rat heart mRNA probed with a eDNA coding for the principal polypeptide of rat liver gap junctions demonstrated a 3.0-kb band. This band was observed only after hybridization and washing using low stringency conditions; high stringency conditions abolished the hybr ..."
Abstract
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Cited by 59 (7 self)
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Abstract. Northern blot analysis of rat heart mRNA probed with a eDNA coding for the principal polypeptide of rat liver gap junctions demonstrated a 3.0-kb band. This band was observed only after hybridization and washing using low stringency conditions; high stringency conditions abolished
unknown title
, 2008
"... Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface ..."
Abstract
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Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface
unknown title
, 2008
"... Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface ..."
Abstract
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Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface
unknown title
, 2008
"... Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface ..."
Abstract
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Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface
unknown title
, 2008
"... Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface ..."
Abstract
- Add to MetaCart
Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface
unknown title
, 2008
"... Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface ..."
Abstract
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Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface
Molecular Cloning of Bovine Papillomavirus Genomes and Comparison of Their Sequence Homologies by Heteroduplex Mapping
, 1982
"... The genomic DNAs of bovine papillomavirus (BPV) type 1, type 2 and type 4 were cloned in pAT153. BPV 1 and BPV2 genomes were cloned using the single HindlII sites of the vector and virus DNAs, and BPV4 was cloned using the single BamHI sites. The orientation of the recombinant DNAs was established b ..."
Abstract
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by restriction enzyme digestion, hybridization and heteroduplex analysis. The results howed that: (i) BPV 1 and BPV2 DNAs are in register and are broadly homologous throughout most of their length when aligned at their single HindlII sites; (ii) depending on the degree of hybridization stringency used, the two
Results 1 - 10
of
100
