E.coli and its effects on cell proliferation

High-level expression of soluble recombinant

PBAD promoter. arabinoseexpression by vectors containing the Tight regulation, modulation, and high-level

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer in the world [1]. There

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Mannans are complex polysaccharides representing one of the major components of hemicellulose, consisting of four types: linear mannan, glucomannan, galactomannan, and galactoglucomanan [1]. Each of these polysaccharides has a β-1,4-linked backbone units including mannose or a combination of glucose and mannose residues, with the presence of α-1,6-linked side-chain substitutions [2]. It was reported that the hydrolysis of these polysaccharides requires several mannan-degrading enzymes, primarily in-cluding β-mannanase (EC 3.2.178), β-mannosidase (EC 3.2.1.25) and β-glucosidase (EC 3.2.1.21). Other enzymes such as α-galactosidase and mannan esterase are required to remove α-galactosyl and O-acetyl side-chain substitu-ent. Among these enzymes, two types of mannan-degrading enzymes are necessary [3]. One endotype, β-mannanase, is responsible for the cleavage of β-1,4-linked mannose residues backbone randomly to generate mannooligosaccharides. Another exotype, β-mannosidase,

Cloning and high-level expression of the glutathione-independent formaldehyde dehydrogenase gene from Pseudomonas putida.

The use of lactose as inducer for the expression of dextransucrase was investigated in high density of Escherichia coli. Different culture conditions including carbon source, nitrogen sources, inorganic salts, inoculum density (OD 600) and lactose concentrations were optimized, and high-level

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