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155
Isolates by Heteroduplex Analysis and Determination of Rifamycin
, 2002
"... This article cites 11 articles, 7 of which can be accessed free at: ..."
Four novel germ-line mutations in the APC gene detected by heteroduplex analysis
, 1994
"... Familial adenomatous polyposis coli (FAP) is an autosomal dominant disease characterised by the presence of 100 or more colorectal adenomatous polyps and the inevitable development, within 10 years, of colon cancer if left untreated. Mutations in the adenomatous polyposis gene {APC) whose mode of ac ..."
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Familial adenomatous polyposis coli (FAP) is an autosomal dominant disease characterised by the presence of 100 or more colorectal adenomatous polyps and the inevitable development, within 10 years, of colon cancer if left untreated. Mutations in the adenomatous polyposis gene {APC) whose mode of action is as a tumour suppressor (1), are primarily responsible for the development of the disease and for many sporadic colon cancers. In 1991, the APC gene was located to chromosome 5q21 (2,3) and was subsequently cloned and sequenced (4-7). The transcribed sequence covers 8535 nucleotides and includes 15 exons. The 15th exon is unusually large with 6500 nucleotides, representing 75 % of the total size of the transcript. More than 60 germ line mutations have been identified in the APC gene (8) with significant clustering in the 5 ' half of exon 15 (9). In this study, we have identified four novel mutations in exon
Capillary electrophoresis-based heteroduplex analysis with a universal heteroduplex generator for detection of point mutations associated with rifampin resistance in tuberculosis
- Clin Chem
, 2001
"... popular scanning method for genetic mutations, uses DNA fragments typically generated by PCR to create homo- and heteroduplex molecules with conformational differences and sequence-dependent electrophoretic profiles. Use of a universal heteroduplex generator (UHG) enhances the subtle variations caus ..."
Abstract
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Cited by 1 (0 self)
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caused by single-base substitutions. Methods: The HDA-UHG slab gel format was modified for an efficient capillary-based method. The effect of staining dyes TOPRO5 and YOPRO1 on the analysis of heteroduplexes was studied, as well as ultraviolet ab-sorbance and laser-induced fluorescence (LIF) detection
Identification of Genetic Variation among Dengue Virus DEN-4 Isolates with Heteroduplex Analysis +
"... Heteroduplex Analysis (HA) was applied for the identification of genetic variation among five DEN-4 isolates. A 398 bp fragment from NS2a-NS2b region was amplified by reverse transcription/polymerase chain reaction (RT/PCR), and the products were first analysed by HA with different strain as referen ..."
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Heteroduplex Analysis (HA) was applied for the identification of genetic variation among five DEN-4 isolates. A 398 bp fragment from NS2a-NS2b region was amplified by reverse transcription/polymerase chain reaction (RT/PCR), and the products were first analysed by HA with different strain
High-throughput mutation detection method to scan BRCA1 and BRCA2 based on heteroduplex analysis by capillary array electrophoresis
- Clin. Chem
, 2004
"... BRCA2 in a large number of samples is hampered by the large sizes of these genes and the scattering of mutations throughout their coding sequences. Automated capillary electrophoresis has been shown to be a powerful system to detect mutations by either single-strand conformation polymorphism or hete ..."
Abstract
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Cited by 3 (2 self)
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or heteroduplex analysis (HA). Methods: We investigated the adaptation of gel-based HA of BRCA1 and BRCA2 to a fluorescent multicapil-lary platform to increase the throughput of this tech-nique. We combined multiplex PCR, three different fluorescent labels, and HA in a 16-capillary DNA se-quencer and tested 57
118 Dengue Bulletin – Vol 26, 2002 Identification of Genetic Variation among Dengue Virus DEN-4 Isolates with Heteroduplex Analysis+
"... Heteroduplex Analysis (HA) was applied for the identification of genetic variation among five DEN-4 isolates. A 398 bp fragment from NS2a-NS2b region was amplified by reverse transcription/polymerase chain reaction (RT/PCR), and the products were first analysed by HA with different strain as referen ..."
Abstract
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Heteroduplex Analysis (HA) was applied for the identification of genetic variation among five DEN-4 isolates. A 398 bp fragment from NS2a-NS2b region was amplified by reverse transcription/polymerase chain reaction (RT/PCR), and the products were first analysed by HA with different strain
Capillary and Microchip Electrophoresis for Rapid Detection of Known Mutations by Combining Allele-specific DNA Amplification with Heteroduplex Analysis
, 2001
"... ..."
Heteroduplex and protein truncation analysis
"... salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16:1215. (31) Ozcelik H, Antebi YJ, Cole DE, Andrulis IL. ..."
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salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16:1215. (31) Ozcelik H, Antebi YJ, Cole DE, Andrulis IL.
METHODS Direct Genomic Multiplex PCR for BRCA1 and Application to Mutation Detection by Single-Strand
, 2016
"... Direct genomic multiplex PCR forBRCA1 and application to mutation detection by single-strand conformation and heteroduplex analysis ..."
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Direct genomic multiplex PCR forBRCA1 and application to mutation detection by single-strand conformation and heteroduplex analysis
Results 1 - 10
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155
