About 10 μM of proteins were freshly prepared in 10 mM potassium phosphate buffer containing 50 mM (NH4)2SO4 at pH 7.4. CD experiments were carried out on a JASCO J-815 circular dichroism spectrometer using a quartz cuvette with a path length of 1 mm at room temperature and scanned from 190 to 260

Abstract We present a theoretical analysis of circular dichroism and bire-fringence in unconventional BCS superconductors with appropriate broken symmetries. We show that for the effect to exist, that in addition to broken time-reversal and 2D parity symmetries, it is necessary to take into account

ABSTRACT: In this paper we present the method of theoretical modelling of circular dichroism shape of crystals for which the circular dichroism is not measurable. We use-quartz as an example of these crystals. The theoretical model of circular dichroism shape is based on the analytical

Amongst the existing techniques for characterizing electronic structure and magnetic interactions, L edge X-ray Absorption Spectroscopy (XAS) and X-ray Magnetic Circular Dichroism (XMCD) are receiving an increasing interest Motivated by several reasons: I) The elemental and orbital selectivity

solubilized in SDS solutions was studied by circular dichroism (CD) spectroscopy and Xuorescence spectroscopy of the single tryptophan residue of this peptide. The results show that the peptide adopts an α-helical conformation or aggregated β-sheet depending on the peptide-to-SDS ratio used. The results

conventional circular dichroism spectroscopy: A comparison

this paper, we are interested in the theory of the circular dichroism in isotropic media. It is well-known that the propagation of the left or right handed circularly polarized electromagnetic waves (LHCP or RHCP) in optically active media is different. If the molecule absorbs at the frequency

Using the semiclassical approach for the description of the propagation of the electromagnetic waves in optically active isotropic media we derive a new formula for the circular dichroism parameter. The theory is based on the idea of the time damped electromagnetic wave interacting

in chloroplasts

Background: Superoxide generated by non-phagocytic NADPH oxidases (NOXs) is of growing importance for physiology and pathobiology. The calcium binding domain (CaBD) of NOX5 contains four EF-hands, each binding one calcium ion. To better understand the metal binding properties of the 1st and 2nd EF-hands, we characterized the N-terminal half of CaBD (NCaBD) and its calcium-binding knockout mutants. Results: The isothermal titration calorimetry measurement for NCaBD reveals that the calcium binding of two EF-hands are loosely associated with each other and can be treated as independent binding events. However, the Ca2+ binding studies on NCaBD(E31Q) and NCaBD(E63Q) showed their binding constants to be 6.5 × 105 and 5.0 × 102 M-1 with ΔHs of-14 and-4 kJ/mol, respectively, suggesting that intrinsic calcium binding for the 1st non-canonical EF-hand is largely enhanced by the binding of Ca2+ to the 2nd canonical EF-hand. The fluorescence quenching and CD spectra support a conformational change upon Ca2+ binding, which changes Trp residues toward a more non-polar and exposed environment and also increases its a-helix secondary structure content. All measurements exclude Mg2+-binding in NCaBD. Conclusions: We demonstrated that the 1st non-canonical EF-hand of NOX5 has very weak Ca2+ binding affinity compared with the 2nd canonical EF-hand. Both EF-hands interact with each other in a cooperative manner to enhance their Ca2+ binding affinity. Our characterization reveals that the two EF-hands in the N-terminal NOX5 are Ca2+ specific.