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Site-directed mutagenesis of UDP-galactopyranose mutase reveals a critical role for the active-site, conserved arginine residues

by Jennifer M. Chad, Karunan Partha Sarathy, Todd D. Gruber, Eshwari Addala, Laura L. Kiessling, David A. R. S - Biochemistry 2007
"... ABSTRACT: The flavoenzyme UDP-galactopyranose mutase (UGM) is a mediator of cell wall biosynthesis in many pathogenic microorganisms. UGM catalyzes a unique ring contraction reaction that results in the conversion of UDP-galactopyranose (UDP-Galp) to UDP-galactofuranose (UDP-Galf). UDP-Galf is an es ..."
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that Arg174 is not. Molecular dynamics simulations indicate that closed UGM conformations can be accessed in which this residue contacts the pyrophosphoryl group of the UDP-Gal substrates. These results provide strong evidence that the mobile loop, noted in all the reported crystal structures, must move

Chemical mechanism of UDP-galactopyranose mutase from Trypanosoma cruzi: a potential drug target against Chagas' disease. PLoS One 2012; 7(3): e32918

by Ana Lisa Valenciano, Karina Kizjakina, Jun Qi, Pablo Sobrado
"... UDP-galactopyranose mutase (UGM) is a flavoenzyme that catalyzes the conversion of UDP-galactopyranose to UDP-galactofuranose, the precursor of galactofuranose (Galf). Galf is found in several pathogenic organisms, including the parasite Trypanosoma cruzi, the causative agent of Chagas ’ disease. Ga ..."
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UDP-galactopyranose mutase (UGM) is a flavoenzyme that catalyzes the conversion of UDP-galactopyranose to UDP-galactofuranose, the precursor of galactofuranose (Galf). Galf is found in several pathogenic organisms, including the parasite Trypanosoma cruzi, the causative agent of Chagas ’ disease

Contributions of Unique Active Site Residues of Eukaryotic UDP- Galactopyranose Mutases to Substrate Recognition and Active Site Dynamics

by Isabel Da Fonseca, Insaf A. Qureshi, Ritcha Mehra-chaudhary, Karina Kizjakina, John J. Tanner, Pablo Sobrado
"... *S Supporting Information ABSTRACT: UDP-galactopyranose mutase (UGM) catalyzes the interconversion between UDP-galactopyranose and UDP-galactofuranose. Absent in humans, galactofuranose is found in bacterial and fungal cell walls and is a cell surface virulence factor in protozoan parasites. For the ..."
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*S Supporting Information ABSTRACT: UDP-galactopyranose mutase (UGM) catalyzes the interconversion between UDP-galactopyranose and UDP-galactofuranose. Absent in humans, galactofuranose is found in bacterial and fungal cell walls and is a cell surface virulence factor in protozoan parasites

Insights into the Activity and Specificity of Trypanosoma cruzi trans- Sialidase from Molecular Dynamics Simulations

by Richard A. Bryce
"... which catalyzes the transfer or hydrolysis of terminal sialic acid residues, is crucial to the development and proliferation of the T. cruzi parasite and thus has emerged as a potential drug target for the treatment of Chagas disease. We here probe the origin of the observed preference for the trans ..."
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synthetic donor substrate, 4-methylumbelliferyl α-D-acetylneuraminide. For this reason, we conducted molecular dynamics simulations of TcTS following its sialylation by the substrate to examine the behavior of the asialyl leaving group by the protein. These simulations indicate that, where lactose
Results 1 - 4 of 4