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"... Motivation: Clustering sequences of a full-length cDNA library into alternative splice form candidates is a very important problem. Results: We developed a new efficient algorithm to cluster sequences of a full-length cDNA library into alternative splice form candidates. Current clustering algorithm ..."
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Motivation: Clustering sequences of a full-length cDNA library into alternative splice form candidates is a very important problem. Results: We developed a new efficient algorithm to cluster sequences of a full-length cDNA library into alternative splice form candidates. Current clustering
The past, present, and future of immune repertoire biology—the rise of next-generation repertoire analysis. Front Immunol 4
, 2013
"... T and B cell repertoires are collections of lymphocytes, each characterized by its antigenspecific receptor. We review here classical technologies and analysis strategies developed to assess immunoglobulin (IG) and T cell receptor (TR) repertoire diversity, and describe recent advances in the field ..."
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to quantify the expression of the human TRAV and TRBV repertoires based on hybridization with gene specific primers coated plates. The cDNA from PBMC extracted RNA are ligated to a universal adaptor which allows for a global amplification of all TRAV or TRBV cDNAs. The PCR products are then transferred onto
