In this paper we describe the major elements of MIT Lincoln Laboratory’s Gaussian mixture model (GMM)-based speaker verification system used successfully in several NIST Speaker Recognition Evaluations (SREs). The system is built around the likelihood ratio test for verification, using simple

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A simple procedure for cloning and stable insertion of foreign genes into the chromosomes of gram-negative eubacteria was developed by combining in two sets of plasmids (i) the transposition features of Tn1O and TnS; (ii) the resistances to the herbicide bialaphos, to mercuric salts and organomercurial compounds, and to arsenite, and (iii) the suicide delivery properties of the R6K-based plasmid pGP704. The resulting constructions contained unique NotI or SfiI sites internal to either the Tn1O or the TnS inverted repeats. These sites were readily used for cloning DNA fragments with the help of two additional specialized cloning plasmids, pUC18Not and pUC18Sfi. The newly derived constructions could be maintained only in donor host strains that produce the R6K-specified protein, which is an essential replication protein for R6K and plasmids derived therefrom. Donor plasmids containing hybrid transposons were transformed into a specialized Xpir lysogenic Escherichia coli strain with a chromosomally integrated RP4 that provided broad-host-range conjugal transfer functions. Delivery of the donor plasmids into selected host bacteria was accomplished through mating with the target strain. Transposition of the hybrid transposon from the delivered suicide plasmid to a replicon in the target cell was mediated by the cognate transposase encoded on the plasmid at a site external to the transposon. Since the transposase function was not maintained in target cells, such cells were not immune to further transposition rounds. Multiple insertions in the same strain are therefore only limited by the availability of distinct selection

the mission of improving DoD’s understanding of the national security implications of the Information Age. Focusing upon improving both the state of the art and the state of the practice of command and control, the CCRP helps DoD take full advantage of the opportunities afforded by emerging technologies. The CCRP pursues a broad program of research and analysis in information superiority, information operations, command and control theory, and associated operational concepts that enable us to leverage shared awareness to improve the effectiveness and efficiency of assigned missions. An important aspect of the CCRP program is its ability to serve as a bridge between the operational, technical, analytical, and educational communities. The CCRP provides leadership for the command and control research community by: n n

The National Research Center on the Gifted and Talented (NRC/GT) is funded under the

The National Research Center on the Gifted and Talented (NRC/GT) is funded under

ECFP into the ROSA26 locus

, being too time consuming. Several other parameters have been used without com-plete validation, however: direct immunoassay of free T with a labeled T analog (aFT), calculation of free T (FT) from total T and immuno-assayed SHBG concentrations (iSHBG), and the free androgen index (FAI 5 the ratio 100T

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Research and Improvement, United States Department of Education. The Directorate of the NRC/GT serves as the administrative unit and is located at