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Characterization of T-DNA Insertion Sites in Arabidopsis thaliana and the Implications for Saturation Mutagenesis
"... A key component of a sound functional genomics infrastructure is the availability of a knockout mutant for every gene in the genome. A fruitful approach to systematically knockingout genes in the plant Arabidopsis thaliana has been the use of transferred-DNA (T-DNA) from Agrobacterium tumefaciens as ..."
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as an insertional mutagen. One of the assumptions underlying the use of T-DNA as a mutagen is that the insertion of these DNA elements into the Arabidopsis genome occurs at randomly selected locations. We have directly investigated the distribution of T-DNA insertions sites in populations of transformed Arabidopsis
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"... Objectives and assessment of the project The objective of this project was to develop a biological and bioinformatic resource making it possible to obtain data of genomic sequences which flank the ADN-T of the mutants affected in genes of selected sequences and thus to facilitate their identificatio ..."
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their identifications. These genomic labels are also named FST for "Flanking Sequence Tag". This project based on a first biological resource: a collection of mutants of T-DNA insertion of Arabidopsis thaliana generated by the INRA-GAP of Versailles, composed of 55 000 primary transforming plants and managed
