Abstract. In this paper, a novel method for diagnosing lymphatic tissue tumors is presented. Microscopic specimen images are analyzed for extracting and characterizing malignant cells. A case-based reasoning approach is followed for classifying morphologic and densitometric cell features so

two images (the reference and sensed images). The reviewed approaches are classified according to their nature (areabased and feature-based) and according to four basic steps of image registration procedure: feature detection, feature matching, mapping function design, and image transformation

The purpose of this chapter is to present a survey of recent publications concerning medical image registration techniques. These publications will be classified according to a model based on nine salient criteria, the main dichotomy of which is extrinsic versus intrinsic methods The statistics

Cluster analysis is the automated search for groups of related observations in a data set. Most clustering done in practice is based largely on heuristic but intuitively reasonable procedures and most clustering methods available in commercial software are also of this type. However

cultivation.of individual microbial cells without Phylogenetic identification and in situ detection

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hundred have been reported in the literature, have serious limitations for many kinds of biological studies. Chief among these is the exclusion of their use for the production of human virus vaccines. This limitation is based on the supposition that such hetero-ploid cell lines, whether of normal

, and heart. Access to the central nervous system and ganglia via translocation along axons and dendrites of neurons has also been observed. NSPs penetrating the skin distribute via uptake into lymphatic channels. Endocytosis and biokinetics are

We have previously demonstrated that human peripheral blood low density mononuclear cells cultured in granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 develop into dendritic cells (DCs) that are extremely efficient in presenting soluble antigens to T ceils. To identify the mechanisms responsible for efficient antigen capture, we studied the endocytic capacity of DCs using fluorescein isothiocyanate--dextran, horseradish peroxidase, and lucifer yellow. We found that DCs use two distinct mechanisms for antigen capture. The first is a high level of fluid phase uptake via macropinocytosis. In contrast to what has been found with other cell types, macropinocytosis in DCs is constitutive and allows continuous internalization of large volumes of fluid. The second mechanism of capture is mediated via the mannose receptor (MR), which is expressed at high levels on DCs. At low ligand concentrations, the MR can deliver a large number of ligands to the cell in successive rounds. Thus, while macropinocytosis endows DCs with a high capacity, nonsaturable mechanism for capture of any soluble antigen, the MR gives an extra capacity for antigen capture with some degree of selectivity for non-self molecules. In addition to their high endocytic capacity, DCs from GM-CSF+ IL-4-dependent cultures are characterized by the presence of a large intracellular compartment that contains high levels of