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in 40 ml of AGK 200 [10 mM Hepes (pH
"... PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY9 ..."
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PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY
in 40 ml of AGK 200 [10 mM Hepes (pH
"... PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY9 ..."
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PRP24 fragment or a 1.4-kb Apa I–Sal I PRP24-Pya fragment (13). Both constructs overexpressed Prp24p when transformed into PRY98 as determined by Western blotting with anti-Prp24 polyclonal antibodies. 19. Three liters of each strain, PRY115 (PRY98 with pG1-PRP24 as sole PRP24 gene) and PRY116 (PRY
Expression of Drosophila adenosine deaminase in immune cells during inflammatory response
"... Details of design and production of AGFP reporter by homologous recombination Materials and methods Design of homologous recombination construct and cloning: We used Drosophila yw strain genomic DNA to amplify three fragments. Using primer F1 5´-gcg gcc gcc atg aaa tcc aca tca agg gg-3 ´ (introducin ..."
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Cited by 4 (1 self)
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acg gtt ggt ggc ag-3 ´ (introducing NheI site to 5 ´ terminus) and primer R3new2 5´- ggg ccc ata tct ctt cct tct tga cgg-3 ´ (introducing ApaI site to 5 ´ terminus) we amplified a 2.2-kb fragment 3 including a short part of the ADGF-B gene and the upstream sequence. A 6-kb fragment 4 was amplified
Supplemental Experimental Procedures
"... To create the Fz4CKOAP targeting construct, loxP sites were inserted (1) within the first exon at the Xma I site 191 bp 5 ’ of the initiator methionine codon, and (2) 3 ’ of an SV40 polyadenylation signal that was inserted immediately 3 ’ of the 3 ’ UTR. EcoR I sites were inserted adjacent to each l ..."
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). In the targeting vector, the 5 ’ arm extended to an Apa I site 7.1 kb upstream of the 5 ’ loxP site, and the 3 ’ arm extended to a Hind III site 3.7 kb downstream of the Fz4 3 ’ UTR. To create the NdpAP knock-in targeting construct, the AP coding region followed by a rabbit beta-globin 3 ’ UTR was inserted
Tol2 transposon-mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo.
- Dev. Dyn.
, 2004
"... We have used the Tol2 transposable element to design and perform effective enhancer trapping in zebrafish. Modified transposon DNA and transposase RNA were delivered into zebrafish embryos by microinjection to produce heritable insertions in the zebrafish genome. The enhancer trap construct carries ..."
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Cited by 22 (1 self)
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with another fish from the same family that carries two inserts (lane 10). Line ET11 has two inserts in the same gene only 4 kb apart; therefore, they cannot be separated by out-cross. EGFP, enhanced GFP. usually performed until 5 dpf, we may have overlooked ET lines with only late (after 5 dpf) expression
