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Manipulation of the Porcine Epidemic Diarrhea Virus Genome Using Targeted RNA Recombination

by Chunhua Li, Zhen Li, Yong Zou, Oliver Wicht, Peter J. M. Rottier , 2013
"... Porcine epidemic diarrhea virus (PEDV) causes severe economic losses in the swine industry in China and other Asian countries. Infection usually leads to an acute, often lethal diarrhea in piglets. Despite the impact of the disease, no system is yet available to manipulate the viral genome which has ..."
Abstract - Cited by 1 (0 self) - Add to MetaCart
has severely hampered research on this virus until today. We have established a reverse genetics system for PEDV based on targeted RNA recombination that allows the modification of the 39-end of the viral genome, which encodes the structural proteins and the ORF3 protein. Using this system, we deleted

Analysis of secondsite revertants of a murine coronavirus nucleocapsid protein deletion mutant and construction of nucleocapsid protein mutants by targeted RNA recombination

by D Peng, C A Koetzner, P S Masters, Ding Peng, Cheri A. Koetzner, Paul S. Masters - Journal of Virology , 1995
"... coronavirus nucleocapsid protein deletion mutant and construction of nucleocapsid protein mutants by targeted RNA recombination. ..."
Abstract - Cited by 10 (4 self) - Add to MetaCart
coronavirus nucleocapsid protein deletion mutant and construction of nucleocapsid protein mutants by targeted RNA recombination.

Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination

by Cheri A. Koetzner, Monica M. Parker, Cynthia S. Ricard, Lawrence S. Sturman, Paul S. Masters , 1992
"... The genetic characterization of a nucleocapsid (N) protein mutant of the coronavirus mouse hepatitis virus (MHIV) is described. The mutant, Albany 4 (Alb4), is both temperature sensitive and thermolabile. Analysis of the progeny of a mixed infection showed that the defective Alb4 allele is recessive ..."
Abstract - Cited by 35 (10 self) - Add to MetaCart
of these properties of Alb4 made it ideal for use as a recipient in a targeted RNA recombination experiment in which the deletion in Alb4 was repaired by recombination with synthetic RNA7, the smallest MHV subgenomic mRNA. Progeny from a cotransfection of Alb4 genomic RNA and synthetic RNA7 were selected for thermal

Optimization of targeted RNA recombination and mapping of a novel nucleocapsid gene mutation in the coronavirus mouse hepatitis virus

by Paul S. Masters, Cheri A. Koetzner, Cheryl A. Kerr, Yong Heo - J , 1994
"... We have recently described a method of introducing site-specific mutations into the genome of the coronavirus mouse hepatitis virus (MHV) by RNA recombination between cotransfected genomic RNA and a synthetic subgenomic mRNA (C. A. Koetzner, M. M. Parker, C. S. Ricard, L. S. Sturman, and P. S. Maste ..."
Abstract - Cited by 39 (10 self) - Add to MetaCart
able to greatly increase the efficiency of targeted recombination in this process by using a synthetic defective interfering (DI) RNA in place of RNA7. The frequency of recombination is sufficiently high that, with Alb4 as the recipient, recombinants can be directly identified without using thermal

A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res

by Michaelw. Pfaffl
"... Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as ..."
Abstract - Cited by 1088 (4 self) - Add to MetaCart
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable

Model-Based Analysis of Oligonucleotide Arrays: Model Validation, Design Issues and Standard Error Application

by Cheng Li, Wing Hung Wong , 2001
"... Background: A model-based analysis of oligonucleotide expression arrays we developed previously uses a probe-sensitivity index to capture the response characteristic of a specific probe pair and calculates model-based expression indexes (MBEI). MBEI has standard error attached to it as a measure of ..."
Abstract - Cited by 775 (28 self) - Add to MetaCart
of accuracy. Here we investigate the stability of the probe-sensitivity index across different tissue types, the reproducibility of results in replicate experiments, and the use of MBEI in perfect match (PM)-only arrays. Results: Probe-sensitivity indexes are stable across tissue types. The target gene

miRBase: microRNA sequences, targets and gene nomenclature

by Sam Griffiths-jones, Russell J. Grocock, Stijn Van Dongen, Alex Bateman, Anton J. Enright - Nucleic Acids Res , 2006
"... nomenclature ..."
Abstract - Cited by 399 (5 self) - Add to MetaCart
nomenclature

A mammalian microRNA expression atlas based on small RNA library sequencing.

by Pablo Landgraf , Mirabela Rusu , Robert Sheridan , Alain Sewer , Nicola Iovino , Alexei Aravin , Sé Bastien Pfeffer , Amanda Rice , Alice O Kamphorst , Markus Landthaler , Carolina Lin , Nicholas D Socci , Leandro Hermida , Valerio Fulci , Sabina Chiaretti , Robin Foà , Julia Schliwka , Uta Fuchs , Astrid Novosel , Roman-Ulrich Mü , Bernhard Schermer , Ute Bissels , Jason Inman , Quang Phan , David B Weir , Ruchi Choksi , Gabriella De Vita , Daniela Frezzetti , Hans-Ingo Trompeter , Veit Hornung , Grace Teng , Gunther Hartmann , Miklos Palkovits , Roberto Di Lauro , Peter Wernet , Giuseppe Macino , Charles E Rogler , James W Nagle , Jingyue Ju , F Nina Papavasiliou , Thomas Benzing , Peter Lichter , Wayne Tam , Michael J Brownstein , Andreas Bosio , James J Russo , Chris Sander , Mihaela Zavolan , Thomas Tuschl - Cell, , 2007
"... SUMMARY MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequencecomplementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ sy ..."
Abstract - Cited by 418 (4 self) - Add to MetaCart
SUMMARY MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequencecomplementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ

Asymmetry in the assembly of the RNAi enzyme complex. Cell 115

by Dianne S. Schwarz, György Hutvágner, Tingting Du, Zuoshang Xu, Neil Aronin, Phillip D. Zamore , 2003
"... A key step in RNA interference (RNAi) is assembly of the RISC, the protein-siRNA complex that mediates target RNA cleavage. Here, we show that the two strands of an siRNA duplex are not equally eligible for assembly into RISC. Rather, both the absolute and relative stabilities of the base pairs at t ..."
Abstract - Cited by 405 (6 self) - Add to MetaCart
A key step in RNA interference (RNAi) is assembly of the RISC, the protein-siRNA complex that mediates target RNA cleavage. Here, we show that the two strands of an siRNA duplex are not equally eligible for assembly into RISC. Rather, both the absolute and relative stabilities of the base pairs

Ratio-Based Decisions and the Quantitative Analysis of cDNA Microarray Images

by Yidong Chen, Edward R. Dougherty, Michael L. Bittner , 1997
"... Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation ..."
Abstract - Cited by 366 (28 self) - Add to MetaCart
Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image
Results 1 - 10 of 20,851